Abstract

In this study, poly (lactic-co-glycolic acid) (PLGA) microparticles loaded with cannabidiol (CBD) were synthesized (PLGA@CBD microparticles) and embedded up to 10 wt% in a chondroitin sulfate/polyvinyl alcohol hydrogel matrix. In vitro chemical, physical, and biological assays were carried out to validate the potential use of the modified hydrogels as biomaterials. The microparticles had spherical morphology and a narrow range of size distribution. CBD encapsulation efficiency was around 52%, loading was approximately 50%. Microparticle addition to the hydrogels caused minor changes in their morphology, FTIR and thermal analyses confirmed these changes. Swelling degree and total porosity were reduced in the presence of microparticles, but similar hydrophilic and degradation in phosphate buffer solution behaviors were observed by all hydrogels. Rupture force and maximum strain at rupture were higher in the modified hydrogels, whereas modulus of elasticity was similar across all materials. Viability of primary human dental pulp cells up to 21 days was generally not influenced by the addition of PLGA@CBD microparticles. The control hydrogel showed no antimicrobial activity against Staphylococcus aureus, whereas hydrogels with 5% and 10% PLGA@CBD microparticles showed inhibition zones. In conclusion, the PLGA@CBD microparticles were fabricated and successfully embedded in a hydrogel matrix. Despite the hydrophobic nature of CBD, the physicochemical and morphological properties were generally similar for the hydrogels with and without the CBD-loaded microparticles. The data reported in this study suggested that this original biomaterial loaded with CBD oil has characteristics that could enable it to be used as a scaffold for tissue/cellular regeneration.

Graphical Abstract

中文翻译：

---

嵌入多孔水凝胶基质中的载有大麻二酚的微粒，用于生物医学应用

摘要

在这项研究中，合成了负载大麻二酚 (CBD) 的聚乳酸-乙醇酸 (PLGA) 微粒（PLGA@CBD 微粒），并将其嵌入硫酸软骨素/聚乙烯醇水凝胶基质中，重量百分比高达 10%。进行了体外化学、物理和生物测定，以验证改性水凝胶作为生物材料的潜在用途。微粒具有球形形态和窄范围的尺寸分布。 CBD 封装效率约为 52%，负载量约为 50%。添加微粒到​​水凝胶中会导致其形态发生微小变化，FTIR 和热分析证实了这些变化。在微粒存在下，溶胀度和总孔隙率降低，但所有水凝胶都观察到类似的亲水性和在磷酸盐缓冲溶液中的降解行为。改性水凝胶的断裂力和最大断裂应变较高，而所有材料的弹性模量相似。添加 PLGA@CBD 微粒通常不会影响原代人牙髓细胞长达 21 天的活力。对照水凝胶对金黄色葡萄球菌没有抗菌活性，而含有 5% 和 10% PLGA@CBD 微粒的水凝胶则显示出抑制区。总之，PLGA@CBD 微粒被制备并成功嵌入水凝胶基质中。尽管 CBD 具有疏水性，但含有和不含有 CBD 负载微粒的水凝胶的物理化学和形态特性通常相似。这项研究报告的数据表明，这种装载 CBD 油的原始生物材料具有使其能够用作组织/细胞再生支架的特性。

图形概要