Aquaporin (AQP) biomimetic membranes are a coming-of-age technology for water purification. Although several studies have reported aquaporin biomimetic membrane fabrication to date, these membranes show low water flux mainly due to the low porosity and inherently dense structure of the polymeric substrate materials. Herein, we report a ceramic-based aquaporin biomimetic membrane based on anodic aluminum oxide (AAO) as a substrate, which has a uniform porous structure with a high aspect ratio and pore density compared to conventional polymer substrates and exhibits a high water flux of 27.6 ± 3.6 LMH (L m⁻² h⁻¹) and superior membrane selectivity of 0.11 g L⁻¹. Briefly, the AAO substrate was functionalized with amino-silane followed by polydopamine coating, then the AQP vesicles were immobilized on the functionalized AAO substrate surface using an electrokinetic method, and the water rejection performance of the membrane was analyzed in a forward osmosis system. Furthermore, a simple cryodesiccation method is introduced to improve the storage stability and easy transportation of aquaporin membranes, which does not require special environmental conditions to transport or store them.

水通道蛋白 (AQP) 仿生膜是一种成熟的水净化技术。尽管迄今为止已有一些研究报道了水通道蛋白仿生膜的制造，但这些膜显示出低水通量，这主要是由于聚合物基材材料的低孔隙率和固有的致密结构。在此，我们报道了一种以阳极氧化铝（AAO）为基材的陶瓷基水通道蛋白仿生膜，与传统聚合物基材相比，该膜具有均匀的多孔结构，具有高的长径比和孔密度，并表现出27.6的高水通量± 3.6 LMH (L m ^-2  h ^{-1 ) 和 0.11 g L -1}的优异膜选择性。简而言之，用氨基硅烷对AAO基底进行功能化，然后涂覆聚多巴胺，然后使用电动方法将AQP囊泡固定在功能化的AAO基底表面上，并在正向渗透系统中分析膜的截水性能。此外，引入了一种简单的冷冻干燥方法，提高了水通道蛋白膜的储存稳定性和易于运输，不需要特殊的环境条件来运输或储存它们。