Covalent protein conjugation facilitates the study of biological processes and the synthesis of therapeutic biomacromolecules. A method that uses vinyl thianthrenium reagents for the site-selective formation of highly reactive episulfonium species on proteins is demonstrated. These in situ-formed intermediates react with diverse nucleophiles, providing access to protein conjugates in one step without purification.

中文翻译：

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蛋白质表面上的位点选择性表锍形成

共价蛋白缀合有助于生物过程的研究和治疗性生物大分子的合成。展示了一种使用乙烯基锍试剂在蛋白质上选择性形成高反应性表锍物种的方法。这些原位形成的中间体与多种亲核试剂反应，无需纯化即可一步获得蛋白质缀合物。