Wild bananas and their relatives are potentially utilized for pre-breeding due to their genetic diversity, disease resistance, and tolerance to abiotic stress, and other desirable traits. The embryonic suspension culture of wild bananas provides a means to harness this genetic diversity for banana genetic improvement. This paper elucidates the response of different subspecies M. acuminata (ssp. malaccensis, microcarpa, sumatrana, and breviformis) to the induction, growth, and behavior of suspension cultures and their regeneration into plantlets. Different subspecies exhibit varied responses starting from the embryogenic culture induction stage, culture proliferation, to plantlet formation. The highest competence for plant regeneration through somatic embryogenesis was found in ssp. malaccensis, followed by microcarpa, sumatrana, and breviformis. The wild banana embryogenic culture consists of somatic embryos, somatic embryo masses, proembryos, and proembryonic masses, and it proliferates through somatic embryo budding and proembryo proliferation. Maintenance and proliferation of suspension cultures were achieved through subculturing medium-sized cell aggregates (300 to 1000 µm). With an inoculum density of 0.3 g per 30 mL medium, the culture’s proliferation rate reached seven times within 25 d. Embryogenic cultures from the suspensions of ssp. malaccensis and microcarpa were capable of forming somatic embryos upon transfer to a semi-solid somatic embryo development medium and later developed shoots on a semi-solid plant regeneration medium, with conversion efficiencies of 35% and 17%, respectively.

野生香蕉及其近缘植物由于其遗传多样性、抗病性、非生物胁迫耐受性以及其他理想特性，可用于预育种。野生香蕉的胚胎悬浮培养提供了一种利用这种遗传多样性进行香蕉遗传改良的方法。本文阐明了不同亚种 M. acuminata（ssp. malaccensis、microcarpa、sumatrana和breviformis）对悬浮培养物的诱导、生长和行为及其再生为幼苗的反应。不同的亚种从胚胎培养诱导阶段、培养增殖到幼苗形成表现出不同的反应。在 ssp 中发现了通过体细胞胚胎发生进行植物再生的最高能力。malaccensis，其次是microcarpa、sumatrana和breviformis。野生香蕉胚胎发生培养物由体细胞胚、体细胞胚团、原胚和原胚团组成，通过体细胞胚出芽和原胚增殖进行增殖。通过传代中等大小的细胞聚集体（300 至 1000 µm）来实现悬浮培养物的维持和增殖。当接种密度为0.3 g/30 mL培养基时，培养物的增殖率在25 d内达到7倍。来自 ssp 悬浮液的胚胎培养物。malaccensis和microcarpa在转移至半固体体细胞胚胎发育培养基后能够形成体细胞胚，随后在半固体植物再生培养基上发育芽，转化效率分别为 35% 和 17%。