Nanopore sequencing technology has broad application prospects in forensic medicine due to its small size, portability, fast speed, real-time result analysis capabilities, single-molecule sequencing abilities, and simple operation. Here, we demonstrate for the first time that nanopore sequencing platforms can be used to identify individuals in the field. Through scientific and reasonable design, a nanopore MinION MK1B device and other auxiliary devices are integrated into a portable detection box conducive to individual identification at the accident site. Individual identification of 12 samples could be completed within approximately 24 h by jointly detecting 23 short tandem repeat (STR) loci. Through double-blinded experiments, the genotypes of 49 samples were successfully determined, and the accuracy of the STR genotyping was verified by the gold standard. Specifically, the typing success rate for 1150 genotypes was 95.3%, and the accuracy rate was 86.87%. Although this study focused primarily on demonstrating the feasibility of full-process testing, it can be optimistically predicted that further improvements in bioinformatics workflows and nanopore sequencing technology will help enhance the feasibility of Oxford Nanopore Technologies equipment for real-time individual identification at accident sites.

中文翻译：

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通过 MinION 纳米孔测序对现场 STR 分型和人类身份测试进行初步验证

纳米孔测序技术因其体积小、便携、速度快、实时结果分析能力、单分子测序能力、操作简单等特点，在法医学领域具有广阔的应用前景。在这里，我们首次证明纳米孔测序平台可用于识别现场个体。通过科学合理的设计，将纳米孔MinION MK1B装置和其他辅助装置集成到便携式检测箱中，有利于事故现场的个体识别。通过联合检测23个短串联重复（STR）位点，可以在大约24小时内完成12个样本的个体识别。通过双盲实验，成功确定了49份样本的基因型，并通过金标准验证了STR基因分型的准确性。其中，1150个基因型的分型成功率为95.3%，准确率为86.87%。尽管这项研究主要侧重于展示全过程测试的可行性，但可以乐观地预测，生物信息学工作流程和纳米孔测序技术的进一步改进将有助于增强牛津纳米孔技术设备在事故现场进行实时个体识别的可行性。