Efficient extraction of camptothecin (CPT), an anticancer agent from the commercial source Nothapodytes nimmoniana (J. Graham) Mabb in India, is of paramount importance. CPT is present in the highest concentration in the stem portion, and the stem can be readily harvested without uprooting the plant. The fluorescence microscopy mapping of the bark matrix for CPT revealed its presence in a free form within both the outer (epidermal and cortical tissues) and inner (xylem and phloem tissues) sections. The bark matrix primarily consists of cellulose, hemicellulose, and lignin, rendering it woody, rigid, and resistant to efficient solvent penetration for CPT extraction. We proposed a hypothesis that subjecting it to disruption through treatment with hydrolytic enzymes like cellulase and xylanase could enhance solvent diffusion, thereby enabling a swift and effective extraction of CPT.

中文翻译：

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喜树碱酶辅助提取工艺优化及表征

喜树碱 (CPT) 是一种从印度商业来源Nothapodytes nimmoniana (J. Graham) Mabb 中提取的抗癌剂，其有效提取至关重要。喜树碱在茎部分的浓度最高，无需将植物连根拔起即可轻松收获茎。 CPT 树皮基质的荧光显微镜图显示其在外部（表皮和皮质组织）和内部（木质部和韧皮部组织）部分中都以游离形式存在。树皮基质主要由纤维素、半纤维素和木质素组成，使其木质化、坚硬，并且能够抵抗喜树碱提取的有效溶剂渗透。我们提出了一个假设，即通过纤维素酶和木聚糖酶等水解酶对其进行破坏，可以增强溶剂扩散，从而能够快速有效地提取喜树碱。