Burbot populations in the Upper Kootenay watershed, transecting the US-Canada border, are subject to intense monitoring and management after severe declines beginning in the 1970s. Genetic monitoring is a high-priority goal for the East Kootenay Burbot Scientific Working Group, but managers do not currently have a genetic marker panel with enough resolution to understand genetic population structure and family structure in the Upper Kootenay burbot populations. Here, we detail the process of developing and optimizing a cost-effective GTseq panel to aid in the conservation of Upper Kootenay burbot. The final GTseq panel contains 331 markers with sufficient variation to accurately infer population structure and reconstruct pedigrees.

横跨美加边境的上库特尼流域的江鳕种群数量自 20 世纪 70 年代开始严重下降后，受到严格的监测和管理。遗传监测是东库特尼江鳕科学工作组的一个高度优先目标，但管理人员目前没有足够分辨率的遗传标记面板来了解上库特尼江鳕种群的遗传群体结构和科结构。在这里，我们详细介绍了开发和优化具有成本效益的 GTseq 面板的过程，以帮助保护上库特尼江鳕。最终的 GTseq panel 包含 331 个标记，这些标记具有足够的变异，可以准确推断种群结构并重建谱系。