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Correction to “FabR senses long-chain unsaturated fatty acids to control virulence in pathogen Edwardsiella piscicida”
Molecular Microbiology ( IF 3.6 ) Pub Date : 2024-02-16 , DOI: 10.1111/mmi.15238


Shao, S., Zhang, Y., Yin, K., Zhang, Y., Wei, L., & Wang, Q. (2022). FabR senses long-chain unsaturated fatty acids to control virulence in pathogen Edwardsiella piscicida. Molecular Microbiology, 117(4): 737–753. https://doi.org/10.1111/mmi.14869

In the above article, the authors compiled Figure 3n (the interaction between FabR and the promoter of cadB) incorrectly by inadvertently using the same image of the binding of FabR to the promoter of cfa (Figure 3b). The results in Figure 3 were used to validate ChIP-seq data with EMSA. The correct image for Figure 3n is shown below.

The authors sincerely apologize for this error and emphasize that they do not affect the conclusions reported in the paper.

Details are in the caption following the image
FIGURE 3
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Validation of FabR regulon by EMSA, ChIP-qPCR, and qRT-PCR analyses. (a–p) Validation of the ChIP-seq results with EMSAs. The enrichment of each of the FabR-controlled promoters (i.e., cfa (a), fadD (c), glpT (e), sseA (g), lldP (i), ETAE_3163 (k), and cadB (m)) bound by FabR are shown in brackets. EMSAs were performed with purified FabR-MBP, 20 ng of each Cy5-labeled probe, and 20 ng of nonspecific competitor DNA poly(dI:dC). Shifts in the DNA probes were detected without (–) or with the addition of 100 and 200 nM of FabR-MBP protein. B: FabR-bound DNA; U: unbound DNA. The enrichment of FabR-binding at the gyrB (o and p) promoter was used as a control. (q) The relative enrichment of FabR binding to various promoters was analyzed by ChIP-qPCR. The results are presented as the mean ± SD. (n = 3). (r) qRT-PCR analysis of the transcripts of the indicated genes in ΔfabR to WT of cells grown in DMEM. *p < 0.05 (Student's t-test) compared with gyrB enrichment (q) or transcript level (r).


中文翻译:

更正“FabR 感知长链不饱和脂肪酸以控制病原体爱德华氏菌的毒力”

邵 S.、张 Y.、尹 K.、张 Y.、魏 L. 和王 Q. (2022)。 FabR 感知长链不饱和脂肪酸以控制病原体爱德华氏菌的毒力。分子微生物学,117(4):737–753。 https://doi.org/10.1111/mmi.14869

在上述文章中,作者无意中使用了 FabR 与cfa启动子结合的相同图像,错误地编译了图 3n(FabR 与cadB启动子之间的相互作用) (图 3b)。图 3 中的结果用于通过 EMSA 验证 ChIP-seq 数据。图 3n 的正确图像如下所示。

作者对这一错误表示诚挚的歉意,并强调它们并不影响论文中报告的结论。

详细信息位于图片后面的标题中
图3
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通过 EMSA、ChIP-qPCR 和 qRT-PCR 分析验证 FabR 调节子。 (a–p) 使用 EMSA 验证 ChIP-seq 结果。每个 FabR 控制的启动子(即cfa (a)、fadD (c)、glpT (e)、sseA (g)、lldP (i)、ETAE_3163 (k) 和cadB (m))的富集结合FabR 的产品显示在括号中。使用纯化的 FabR-MBP、20 ng 每种 Cy5 标记探针和 20 ng 非特异性竞争 DNA 聚 (dI:dC) 进行 EMSA。在不添加 (-) 或添加 100 和 200 nM FabR-MBP 蛋白的情况下检测到 DNA 探针的变化。 B:FabR 结合 DNA; U:未结合的DNA。gyrB (o 和 p)启动子处 FabR 结合的富集用作对照。 (q) 通过 ChIP-qPCR 分析 FabR 与各种启动子结合的相对富集度。结果以平均值±标准差表示 。 (n  = 3)。 (r) 对 DMEM 中生长的细胞的 Δ fabR至 WT中指定基因的转录本进行 qRT-PCR 分析。 *与gyrB富集 (q) 或转录水平 (r)相比, p  < 0.05(学生t检验)。
更新日期:2024-02-16
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