Purpose

To identify whether follicular environment parameters are associated with mature oocyte quality, embryological and clinical outcomes.

Methods

This retrospective study examined 303 mature oocytes from 51 infertile women undergoing ICSI cycles between May 2018 and June 2021. Exclusion criteria consisted of advanced maternal age (> 36 years old), premature ovarian failure, obesity in women, or use of frozen gametes. Luteal granulosa cells (LGCs) were analyzed for mitochondrial DNA/genomic (g) DNA ratio and vitality. The relationships between hormone levels in the follicular fluid and oocyte features were assessed. Quantitative morphometric measurements of mature oocytes were assessed, and the association of LGC parameters and oocyte features on live birth rate after single embryo transfer was examined.

Results

Results indicated an inverse correlation between the mtDNA/gDNA ratio of LGCs and the size of polar body I (PBI). A 4.0% decrease in PBI size was observed with each one-unit increase in the ratio (p = 0.04). Furthermore, a 1% increase in LGC vitality was linked to a 1.3% decrease in fragmented PBI (p = 0.03), and a 1 ng/mL increase in progesterone levels was associated with a 0.1% rise in oocytes with small inclusions (p = 0.015). Associations were drawn among LGC characteristics, perivitelline space (PVS) debris, cytoplasmic inclusions, PBI integrity, and progesterone levels. Certain dysmorphisms in mature oocytes were associated with embryo morphokinetics; however, live birth rates were not associated with follicular parameters and oocyte quality characteristics.

Conclusion

Follicular markers may be associated with mature oocyte quality features.

中文翻译：

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成熟卵母细胞畸形可能与黄体颗粒细胞的黄体酮水平、线粒体 DNA 含量和活力有关

目的

确定卵泡环境参数是否与成熟卵母细胞质量、胚胎学和临床结果相关。

方法

这项回顾性研究检查了 2018 年 5 月至 2021 年 6 月期间接受 ICSI 周期的 51 名不孕女性的 303 个成熟卵母细胞。排除标准包括高龄产妇（> 36 岁）、卵巢早衰、女性肥胖或使用冷冻配子。分析黄体颗粒细胞 (LGC) 的线粒体 DNA/基因组 (g) DNA 比率和活力。评估了卵泡液中激素水平与卵母细胞特征之间的关系。对成熟卵母细胞的定量形态测量进行了评估，并检查了 LGC 参数和卵母细胞特征与单胚胎移植后活产率的关系。

结果

结果表明 LGC 的 mtDNA/gDNA 比率与极体 I (PBI) 的大小之间呈负相关。比率每增加一个单位，PBI 大小就会减少 4.0% ( p  = 0.04)。此外，LGC 活力增加 1% 与碎片 PBI 减少 1.3% 相关（p  = 0.03），黄体酮水平增加 1 ng/mL 与小内含物卵母细胞增加 0.1% 相关（p  = 0.015）。 LGC 特征、卵周间隙 (PVS) 碎片、细胞质内含物、PBI 完整性和孕酮水平之间存在关联。成熟卵母细胞的某些畸形与胚胎形态动力学有关。然而，活产率与卵泡参数和卵母细胞质量特征无关。

结论

卵泡标志物可能与成熟卵母细胞的质量特征相关。