The redirection of T cells has emerged as an attractive therapeutic principle in B cell non-Hodgkin lymphoma (B-NHL). However, a detailed characterization of lymphoma-infiltrating T cells across B-NHL entities is missing. Here we present an in-depth T cell reference map of nodal B-NHL, based on cellular indexing of transcriptomes and epitopes, T cell receptor sequencing, flow cytometry and multiplexed immunofluorescence applied to 101 lymph nodes from patients with diffuse large B cell, mantle cell, follicular or marginal zone lymphoma, and from healthy controls. This multimodal resource revealed quantitative and spatial aberrations of the T cell microenvironment across and within B-NHL entities. Quantitative differences in PD1⁺ TCF7⁻ cytotoxic T cells, T follicular helper cells or IKZF3⁺ regulatory T cells were linked to their clonal expansion. The abundance of PD1⁺ TCF7⁻ cytotoxic T cells was associated with poor survival. Our study portrays lymphoma-infiltrating T cells with unprecedented comprehensiveness and provides a unique resource for the investigation of lymphoma biology and prognosis.

T 细胞的重定向已成为 B 细胞非霍奇金淋巴瘤 (B-NHL) 的一个有吸引力的治疗原则。然而，B-NHL 实体中淋巴瘤浸润 T 细胞的详细特征却缺失。在这里，我们展示了淋巴结 B-NHL 的深入 T 细胞参考图，基于转录组和表位的细胞索引、T 细胞受体测序、流式细胞术和多重免疫荧光，应用于弥漫性大 B 细胞、套膜患者的 101 个淋巴结细胞、滤泡或边缘区淋巴瘤，以及来自健康对照。这种多模式资源揭示了 B-NHL 实体之间和内部 T 细胞微环境的数量和空间畸变。PD1 ⁺ TCF7 ^-细胞毒性 T 细胞、滤泡辅助 T 细胞或 IKZF3 ⁺调节性 T 细胞的数量差异与其克隆扩张有关。^{PD1 +} TCF7 ^-细胞毒性 T 细胞的丰度与生存率低相关。我们的研究以前所未有的全面性描绘了淋巴瘤浸润性 T 细胞，并为淋巴瘤生物学和预后的研究提供了独特的资源。