In this study, we investigated and confirmed natural lumpy skin disease virus (LSDV) infection in Himalayan yaks (Bos grunniens) in Himachal Pradesh, India, based on clinical manifestations and results of genome detection, antibody detection, virus isolation, and nucleotide sequencing. Subsequent phylogenetic analysis based on complete GPCR, RPO30, and EEV gene sequences revealed that the LSDV isolates from these yaks and local cattle belonged to LSDV subcluster 1.2.1 rather than the dominant subcluster 1.2.2, which is currently circulating in India, suggesting a separate recent introduction. This is the first report of natural LSDV infection in yaks in India, expanding the known host range of LSDV. Further investigations are needed to assess the impact of LSDV infection in yaks.

在本研究中，我们根据临床表现以及基因组检测、抗体检测、病毒分离和核苷酸测序结果，调查并证实了印度喜马偕尔邦喜马拉雅牦牛（ Bos grunniens）的自然结节性皮肤病病毒（LSDV）感染。随后基于完整 GPCR、RPO30 和 EEV 基因序列的系统发育分析表明，从这些牦牛和当地牛身上分离到的 LSDV 属于 LSDV 亚类 1.2.1，而不是目前在印度流行的优势亚类 1.2.2，这表明单独近期介绍。这是印度牦牛自然感染LSDV的首次报道，扩大了LSDV的已知宿主范围。需要进一步研究来评估 LSDV 感染对牦牛的影响。