The liver contributes to lipid metabolism as the hub of fat synthesis. Long non-coding RNAs (lncRNAs) are considered the regulators of cellular processes. Since LncRNA ENSGALG00000021686 (lncRNA 21 686) has been described as a regulator of lipid metabolism, the present study aimed to clarify the role of lncRNA 21 686 in chicken hepatocytes’ lipid metabolism. Thirty-two chickens were divided into four groups and were treated with diets containing different amounts of fat, and the hepatic expression of lncRNA 21 686 and miR-146b along with the levels of proteins involved in the regulation of fat metabolism, lipid indices and oxidative stress were measured. Moreover, primary chicken hepatocytes were transfected with lncRNA 21 686 small interfering RNA or microRNA (miRNA, miR)-146b mimics to measure the consequences of suppressing lncRNA or inducing miRNA expression on the levels of proteins involved in fat metabolism and stress markers. The results showed that the high-fat diet modulated the expression of lncRNA 21 686 and miR-146b (p-value < 0.001). Moreover, there was a significant increase in 1-acyl-sn-glycerol-3-phosphate acyltransferase 2 (AGPAT2) gene expression and protein levels and modulated fat-related markers. Furthermore, the results showed that lncRNA 21 686 suppression reduced the expression of AGPAT2 and its downstream proteins (p-value < 0.05). Overexpression of miR-146b regulated fat metabolism indicator expression. Transfection experiments revealed that lncRNA 21 686 suppression increased miR-146b expression. The findings suggested a novel mechanism containing lncRNA 21 686/miR-146b/AGPAT2 in the regulation of fat metabolism in chicken hepatocytes.

中文翻译：

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LncRNA ENSGALG00000021686 通过 miR-146b/AGPAT2 通路调节鸡肝细胞脂肪代谢

肝脏作为脂肪合成的枢纽，有助于脂质代谢。长非编码 RNA (lncRNA) 被认为是细胞过程的调节因子。由于LncRNA ENSGALG00000021686 (lncRNA 21 686)被描述为脂质代谢的调节因子，本研究旨在阐明lncRNA 21 686在鸡肝细胞脂质代谢中的作用。将 32 只鸡分为 4 组，饲喂不同脂肪含量的饲料，检测肝脏中lncRNA 21 686和 miR-146b 的表达以及参与脂肪代谢、脂质指数和氧化调节的蛋白质水平。测量了压力。此外，用lncRNA 21 686小干扰 RNA 或 microRNA (miRNA, miR ) -146b模拟物转染原代鸡肝细胞，以测量抑制 lncRNA 或诱导 miRNA 表达对参与脂肪代谢和应激标记的蛋白质水平的影响。结果表明，高脂肪饮食调节lncRNA 21 686和miR-146b的表达（p值 < 0.001）。此外，1-酰基-sn-甘油-3-磷酸酰基转移酶 2 ( AGPAT2 ) 基因表达和蛋白质水平以及调节的脂肪相关标志物显着增加。此外，结果表明lncRNA 21 686抑制降低了AGPAT2及其下游蛋白的表达（ p值 < 0.05）。miR-146b的过表达调节脂肪代谢指标的表达。转染实验表明，lncRNA 21 686抑制增加了miR-146b 的表达。研究结果表明， lncRNA 21 686 / miR-146b / AGPAT2调控鸡肝细胞脂肪代谢的新机制。