Background

High temperature stress is an important abiotic factor, which affects tuberization and ultimately causes heavy yield reduction in potato.

Objectives

Identification and characterization of genes associated with tuberization under high temperature stress is essential for future management through biotechnology.

Methodology

Two contrasting potato varieties Kufri Anand (profuse tuber-bearing) versus Kufri Frysona (very less/scanty tuber-bearing, control) were cultivated in aeroponics under high temperature stress, and transcriptomes were analyzed.

Results

Potato cv. Kufri Anand was found superior over control (Kufri Frysona) for tuber yield and its component traits along with root morphology under aeroponics. Transcriptomes of tuber and leaf tissues were analyzed. Statistically significant (p < 0.05) differentially expressed genes (DEGs) were categorised into up-regulated (> 2 log₂ fold change, FC) and down-regulated (< -2 log2 FC) genes. DEGs were annotated by gene ontology and KEGG pathways. A few selected up-regulated genes of both tissues were identified, and phylogeny tree and motif analysis were analysed based on 36 peptide sequences representing 15 selected DEGs in this study. Further, gene expression markers were developed and validated by real time qPCR analysis for the identification of high temperature tolerant genotypes.

Conclusion

A few key genes associated in tuberization under high temperature conditions were heat shock proteins (e.g. 18.5 kDa class I heat shock protein), sugar metabolism (e.g. glucosyltransferase), transcription factor (e.g. WRKY), and phytohormones (e.g. auxin-induced beta-glucosidase). Our study provides an overview of key genes involved in tuberization under high temperature stress in potato cv. Kufri Anand under aeroponics.

中文翻译：

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马铃薯气培高温下结薯相关基因的转录组分析和表征库夫里·阿南德

背景

高温胁迫是影响马铃薯块茎形成的重要非生物因子，最终导致马铃薯大幅减产。

目标

高温胁迫下与块茎形成相关的基因的鉴定和表征对于未来通过生物技术进行管理至关重要。

方法

两个对比的马铃薯品种 Kufri Anand（块茎丰富）与 Kufri Frysona（块茎很少/很少，对照）在高温胁迫下采用气培法进行栽培，并进行了转录组分析。

结果

马铃薯品种发现 Kufri Anand 在气培法下的块茎产量及其组成性状以及根形态方面优于对照（Kufri Frysona）。分析了块茎和叶组织的转录组。具有统计学意义（p  < 0.05）的差异表达基因（DEG）分为上调基因（> 2 log ₂倍变化，FC）和下调基因（< -2 log2 FC）。 DEGs 通过基因本体和 KEGG 通路进行注释。本研究中鉴定了两种组织中一些选定的上调基因，并基于代表 15 个选定 DEG 的 36 个肽序列进行了系统发育树和基序分析。此外，还通过实时 qPCR 分析开发和验证了基因表达标记，以鉴定耐高温基因型。

结论

高温条件下与块茎形成相关的一些关键基因是热休克蛋白（例如18.5 kDa I类热休克蛋白）、糖代谢（例如葡萄糖基转移酶）、转录因子（例如WRKY）和植物激素（例如生长素诱导的β-葡萄糖苷酶） ）。我们的研究概述了马铃薯品种高温胁迫下与块茎形成相关的关键基因。气培法下的 Kufri Anand。