Abstract

Direct organogenesis is an important technique for plant rapid propagation, which is mainly controlled by the balance of auxin and cytokinin. Pinus massoniana (Lamb.) is a perennial tree species with high application value. Previous studies have shown that direct organ regeneration of young P. massoniana is feasible. However, there are few reports on direct organogenesis of adult P. massoniana. This research studied the effects of apical shoot bud disinfection, plant growth regulators of axillary bud induction, and adventitious root induction of adult P. massoniana. The results showed that the survival rate could reach 50% after removing the outer coat scales and soaking in 500.0 mg L⁻¹ carbendazim solution for 10 min, 75% alcohol for 30 s, and 2.0% NaClO with one drop of Tween for 25 min. The addition of 0.2% plant preservative mixture (PPM) to the medium effectively inhibited contamination of endogenous bacteria, and the survival rate was 90%. The suitable medium for axillary bud induction was Murashige and Skoog (MS) basal medium with 6-benzyl aminopurine (6-BA, 2.0 mg L⁻¹) and indolebutyric acid (IBA, 0.1 mg L⁻¹), and the induction rate reached 15%. A single apical shoot bud induced up to seven axillary buds, and the axillary buds grew vigorously. In addition, 6-BA (2.0 mg L⁻¹) was suitable for needle bundle axillary bud induction. Quarter Douglas-fir cotyledon revised medium (DCR; Gupta and Durzan 1985) with naphthaleneacetic acid (NAA, 0.5 mg L⁻¹) and IBA (0.1 mg L⁻¹) was the most suitable for adventitious root induction. This study preliminarily constructed a regeneration system for direct organogenesis of adult P. massoniana, which was expected to provide key technical support for vegetative propagation of excellent breeding materials for adult P. massoniana.

中文翻译：

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成年马尾松顶芽直接器官再生

摘要

直接器官发生是植物快速繁殖的重要技术，其主要受生长素和细胞分裂素的平衡控制。马尾松是多年生树种，具有很高的应用价值。此前的研究表明，幼龄马尾松的直接器官再生是可行的。然而，关于马尾松成虫直接器官发生的报道却很少。本研究研究了顶芽消毒、植物生长调节剂对马尾松成虫腋芽诱导和不定根诱导的影响。结果表明，去除外皮鳞后，用500.0 mg·L ^-1多菌灵溶液浸泡10 min，75%酒精浸泡30 s，2.0% NaClO加1滴吐温浸泡25 min，成活率可达50%。 。培养基中添加0.2%植物防腐剂混合物（PPM）有效抑制内源细菌的污染，存活率为90%。腋芽诱导的适宜培养基为含有6-苄基氨基嘌呤(6-BA, 2.0 mg L ^-1 )和吲哚丁酸(IBA, 0.1 mg L ^-1 )的Murashige and Skoog (MS)基础培养基,诱导率达到15%。单个顶芽可诱发多达7个腋芽，且腋芽生长旺盛。此外，6-BA（2.0 mg L ^-1）适合针束腋芽诱导。含有萘乙酸（NAA，0.5 mg L ^-1）和IBA（0.1 mg L ^-1）的四分之一花旗松子叶改良培养基（DCR；Gupta 和Durzan 1985）最适合不定根诱导。本研究初步构建了马尾松成虫直接器官发生再生体系，有望为马尾松成虫优良育种材料的无性繁殖提供关键技术支撑。