Abstract

Creation of biocompatible coatings for xenogenic materials that can be used to manufacture prosthetic heart valves is an urgent and, unfortunately, still unsolved problem. It is necessary to obtain a biomaterial that would comply with the mechanical characteristics of a human valve and possess antimicrobial properties, which are of critical importance during the first postsurgical days. Biocompatible coatings can be used for this purpose, and it has turned out that detonation nanodiamonds are suitable for their preparation. The developed functional surface of nanodiamonds allows them to adsorb antibiotics; nanodiamonds are nontoxic and do not cause additional calcification. In this study, we have proposed to prepare a composite coating composed of nanodiamonds, lysozyme, and miramistin as broad-spectrum antimicrobial agents. The use of tritium-labeled nanodiamonds has made it possible to study the distribution of nanodiamond–lysozyme complexes after intravenous administration to mice and showed that the majority of the material remains at the place of injection. It has been shown that nanodiamond–lysozyme–miramistin composites exhibit strong antimicrobial activity, while the nanodiamond–miramistin complex shows no toxicity with respect to Staphylococcus aureus. Thus, the nanodiamond–lysozyme–miramistin composite can be used to create coatings for materials of prosthetic heart valves.

中文翻译：

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纳米金刚石-溶菌酶-米拉米斯汀复合材料的制备及其在人工心脏瓣膜中的应用前景

摘要

为异种材料创建可用于制造人工心脏瓣膜的生物相容性涂层是一个紧迫的问题，不幸的是，该问题仍未解决。有必要获得一种符合人体瓣膜机械特性并具有抗菌特性的生物材料，这在术后最初几天至关重要。生物相容性涂层可用于此目的，并且事实证明爆炸纳米金刚石适合其制备。纳米金刚石发达的功能表面使其能够吸附抗生素；纳米金刚石无毒，不会引起额外的钙化。在这项研究中，我们提出制备一种由纳米金刚石、溶菌酶和米拉米丁组成的复合涂层作为广谱抗菌剂。使用氚标记的纳米金刚石使得研究纳米金刚石-溶菌酶复合物在给小鼠静脉注射后的分布成为可能，并表明大部分材料保留在注射位置。研究表明，纳米金刚石-溶菌酶-miramistin复合物表现出很强的抗菌活性，而纳米金刚石-miramistin复合物对金黄色葡萄球菌没有毒性。因此，纳米金刚石-溶菌酶-米拉米斯丁复合材料可用于制造人工心脏瓣膜材料的涂层。