The study aimed to determine the inter and intra-host Bartonella spp. genetic diversity in cats from Chile. ‘Seventy-nine cats’ blood DNA samples qPCR spp. positive were subjected to T-A cloning of spp. partial gene (825 bp), and sequencing by Sanger method. The sequences were submitted to phylogenetic and polymorphism analysis. Thirty-six (45.6%) samples were successfully cloned, generating 118 clones of which 109 showed 99.6%–100% identity with whereas 9 showed 99.8–100% identity with . Haplotype analysis yielded 29 different - haplotypes, one (hap#2) overrepresented in 31 out of 33 cats, and 4 haplotypes, with hap#2 represented in all 3 infected cats. More than one - and haplotypes were identified in individual cats, reporting by first time coinfection by different variants in cats from Chile.

中文翻译：

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智利家猫巴尔通体 rpoB 单倍型的遗传多样性

该研究旨在确定宿主间和宿主内的巴尔通体属。智利猫的遗传多样性。 “七十九只猫”血液 DNA 样本 qPCR spp.阳性者进行 TA 克隆。部分基因（825 bp），桑格法测序。将序列提交给系统发育和多态性分析。成功克隆了 36 个 (45.6%) 样本，产生 118 个克隆，其中 109 个与 99.6%–100% 同一性，9 个与 99.8–100% 同一性。单倍型分析产生了 29 种不同的单倍型，其中一种 (hap#2) 在 33 只猫中的 31 只中出现过多，还有 4 种单倍型，其中 hap#2 在所有 3 只受感染的猫中出现。在个别猫中鉴定出不止一种单倍型，报告称智利猫首次被不同变种同时感染。