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Comparative Evaluation of Two Molecular Multiplex Syndromic Panels with Acute Gastroenteritis
Diagnostic Microbiology and Infectious Disease ( IF 2.9 ) Pub Date : 2024-02-10 , DOI: 10.1016/j.diagmicrobio.2024.116211
Kuenyoul Park , Bo-Moon Shin

We compared the Allplex Gastrointestinal V/B1/B2 Assays and Seeplex Diarrhea V/B1/B2 ACE Detection Assays in patients with acute gastroenteritis (AGE). Of the total 432 specimens, 48.8% and 54.9% samples were positive for any bacterial or viral target using Seeplex and Allplex, respectively ( = 0.002). The overall percent agreement (OPA) between the two panels was >95% and the lowest OPA was 95.4% for CdB. Allplex identified 40 samples positive for , while Seeplex and OBC identified only 27 (67.5%) and 8 (20%), respectively. were detected by assays in six samples, but none were identified using culture. with Seeplex was detected in 70 (16.2%). It remained an informative species in identifying AGE although gene showed only 9.8% positivity. Pathogenic with Allplex could be detected in 35 (8.1%) samples, which could provide valuable information for the diagnosis of AGE.

中文翻译:

两种急性胃肠炎分子多重综合征组的比较评价

我们比较了急性胃肠炎 (AGE) 患者的 Allplex Gastrointestinal V/B1/B2 检测和 Seeplex Diarrea V/B1/B2 ACE 检测检测。使用 Seeplex 和 Allplex,在总共 432 个样本中,分别有 48.8% 和 54.9% 的样本对任何细菌或病毒目标呈阳性 (= 0.002)。两个面板之间的总体一致性百分比 (OPA) > 95%,CdB 的最低 OPA 为 95.4%。Allplex 鉴定出 40 个样本呈阳性,而 Seeplex 和 OBC 分别仅鉴定出 27 个 (67.5%) 和 8 个 (20%)。通过在六个样品中进行测定检测到了这些物质,但没有一个是通过培养来鉴定的。在 70 例 (16.2%) 中检测到了 Seeplex。尽管基因仅显示 9.8% 的阳性率,但它仍然是鉴定 AGE 的信息丰富的物种。Allplex可在35个(8.1%)样本中检测到致病性,这可以为AGE的诊断提供有价值的信息。
更新日期:2024-02-10
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