The lens is an avascular tissue, where epithelial cells (LECs) are the primary living cells. The role of LECs-derived exosomes (LEC-exos) is largely unknown. In our study, we determined the anti-angiogenic role of LEC-exos, manifested as regressed retinal neovascularization (NV) using the oxygen-induced retinopathy (OIR), and reduced choroidal NV size and pathological vascular leakage using the laser-induced choroidal neovascularization (laser-induced CNV). Furthermore, the activation and accumulation of microglia were also restricted by LEC-exos. Based on Luminex multiplex assays, the expressions of chemokines such as SCYB16/CXCL16, MCP-1/CCL2, I-TAC/CXCL11, and MIP 3beta/CCL19 were decreased after treatment with LEC-exos. Transwell assays showed that LEC-exos restricted the migration of the mouse microglia cell line (BV2 cells). After incubation with LEC-exos-treated BV2 cells, human umbilical vein endothelial cells (hUVECs) were collected for further evaluation using tube formation, Transwell assays, and 5-ethynyl-2′-deoxyuridine EDU) assays. Using in vitro experiments, the pro-angiogenic effect of microglia was restricted by LEC-exos. Hence, it was investigated that LEC-exos attenuated ocular NV, which might attribute to the inhibition of microglial activation and accumulation.

中文翻译：

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人晶状体上皮分泌的外泌体通过抑制小胶质细胞活化来减弱眼部血管生成

晶状体是一种无血管组织，其中上皮细胞（LEC）是主要的活细胞。 LEC 衍生的外泌体 (LEC-exos) 的作用很大程度上未知。在我们的研究中，我们确定了 LEC-exos 的抗血管生成作用，表现为使用氧诱导视网膜病变 (OIR) 使视网膜新生血管 (NV) 消退，并使用激光诱导脉络膜新生血管减少脉络膜 NV 大小和病理性血管渗漏（激光诱导的 CNV）。此外，小胶质细胞的激活和积累也受到LEC-exos的限制。根据 Luminex 多重检测，LEC-exos 治疗后，SCYB16/CXCL16、MCP-1/CCL2、I-TAC/CXCL11 和 MIP 3beta/CCL19 等趋化因子的表达下降。 Transwell 检测显示 LEC-exos 限制小鼠小胶质细胞系（BV2 细胞）的迁移。与 LEC-exos 处理的 BV2 细胞孵育后，收集人脐静脉内皮细胞 (hUVEC)，以使用管形成、Transwell 测定和 5-乙炔基-2'-脱氧尿苷 EDU 测定进行进一步评估。通过体外实验，小胶质细胞的促血管生成作用受到 LEC-exos 的限制。因此，研究发现 LEC-exos 减弱眼部 NV，这可能归因于抑制小胶质细胞的激活和积累。