2,3-Butanediol (2,3-BD) is a high-value chemical compound with various applications in the industry. Conversion of agricultural wastes or by-products into 2,3-BD via microbial fermentation processes would be an environmentally friendly approach to reduce waste and sustain the production of biotechnologically important chemicals. Therewithal, sterilization of waste feedstock, which is usually required before fermentation to prevent any contamination, is not a cost-effective step because of energy consuming. The present study was performed to produce 2,3-BD from molasses using immobilized cells of locally isolated and nonpathogenic BD43 strain under non-sterile fermentation conditions. Sodium alginate was used as entrapping agent for cell immobilization. The highest production of 2,3-BD was obtained at temperature of 55 ⁰C, pH 7, molasses concentration of 100 mL/L, bead number of 150 beads/100 mL, 5 mm bead size and incubation time of 24 h. In optimized non-sterile batch fermentation of 100 mL/L molasses (48.3 g sugar), 31.6 g/L of 2,3-BD was obtained with a yield of 0.65 g/g. Non-sterile fermentation of molasses using immobilized cells of in the production of 2,3-BD was investigated for the first time. The study indicates that thermophilic BD43 is a suitable candidate for non-sterile and energy-saving processes to convert waste feedstock to value-added chemicals.

中文翻译：

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将废物转化为生物燃料：使用地衣芽孢杆菌 BD43 固定化细胞对糖蜜进行非无菌发酵，以高效生产 2,3-丁二醇

2,3-丁二醇 (2,3-BD) 是一种高价值化合物，在工业中具有多种应用。通过微生物发酵过程将农业废物或副产品转化为 2,3-BD 将是一种环境友好的方法，可减少废物并维持生物技术上重要的化学品的生产。此外，发酵前通常需要对废弃原料进行灭菌以防止任何污染，但由于消耗能量，这不是一个具有成本效益的步骤。本研究使用局部分离的非致病性 BD43 菌株的固定化细胞在非无菌发酵条件下从糖蜜生产 2,3-BD。海藻酸钠用作细胞固定的包封剂。2,3-BD 的最高产量在温度 55℃、pH 7、糖蜜浓度 100 mL/L、珠数 150 珠/100 mL、珠尺寸 5 mm 和孵育时间 24 h 时获得。在100 mL/L糖蜜（48.3 g糖）的优化非无菌分批发酵中，获得了31.6 g/L 2,3-BD，产量为0.65 g/g。首次研究了使用固定化细胞进行糖蜜非无菌发酵生产2,3-BD。研究表明，嗜热 BD43 是非无菌和节能工艺的合适候选者，可将废物原料转化为增值化学品。