This study elaborates on the utilization of biomass for the pilot-scale production of glucose oxidase from and their subsequent application in the valorization of biomass via bio-Fenton oxidation for fermentable sugar production. Optimized medium parameters (0.13 % of yeast extract, 3.1 % of CaCO, 0.7 mg of biotin, and 0.06 % of triton X), and operational parameters (120 RPM of agitation, 2 VVM of aeration, 11 % inoculum size, and 80 h of incubation) resulting in 1261±2.08 U/mL of glucose oxidase production in 25 L stirred tank reactor. A potential cost-effective and environmentally friendly method for fermentable sugar production was devised, utilizing biomass in conjunction with the bio-Fenton oxidation by glucose oxidase. Through a systematic examination, the optimum process conditions were determined by response surface methodology, including the quantity of hydrogen peroxide and FeSO of 25.5:1 ratio, the reaction duration of 13 h, and biomass concentration of 7.5 %. The maximum fermentable sugar concentration optimized was 595±0.62 mg/g of biomass by the bio-Fenton oxidation. These findings illustrate the feasibility of employing bio-Fenton chemistry as an effective pretreatment approach to enhance the accessibility of biomass for fermentable sugar conversion as well as platform chemical production.

中文翻译：

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基于真菌葡萄糖氧化酶的生物芬顿氧化作为木麻黄生物质预处理工艺以获得可发酵糖的中试生产和应用

本研究详细阐述了利用生物质进行葡萄糖氧化酶的中试规模生产，以及随后通过生物芬顿氧化生产可发酵糖来提高生物质价值的应用。优化的培养基参数（0.13% 酵母提取物、3.1% CaCO3、0.7 mg 生物素和 0.06% triton X）和操作参数（120 RPM 搅拌、2 VVM 通气、11% 接种量和 80 小时）孵化）导致 25 L 搅拌釜反应器中葡萄糖氧化酶产量为 1261±2.08 U/mL。设计了一种潜在的经济有效且环境友好的可发酵糖生产方法，利用生物质与葡萄糖氧化酶的生物芬顿氧化相结合。通过系统考察，采用响应面法确定了最佳工艺条件：过氧化氢与FeSO的用量比例为25.5:1，反应时间为13 h，生物质浓度为7.5%。通过生物芬顿氧化优化的最大可发酵糖浓度为595±0.62mg/g生物质。这些发现说明了采用生物芬顿化学作为有效预处理方法的可行性，以提高生物质可发酵糖转化以及平台化学品生产的可及性。