Testicular hyperthermia has been noted in men who work in high ambient temperatures. Scrotal temperatures above the normal range caused germ cell loss in the testes and resulted in male subfertility. In adult male rats, exercising at a higher environmental temperature (36 °C with relative humidity of 50%, 52 min) caused exertional heat stroke (EHS) characterized by scrotal hyperthermia, impaired sperm quality, dysmorphology in testes, prostates and bladders, and erectile dysfunction. Here, we aim to ascertain whether hyperbaric oxygen preconditioning (HBOP: 100% O at 2.0 atm absolute [ATA] for 2 h daily for 14 days consequently before the onset of EHS) is able to prevent the problem of EHS-induced sterility, testes, prostates, and bladders dysmorphology and erectile dysfunction. At the end of exertional heat stress compared to normobaric air (NBA or non-HBOP) rats, the HBOP rats exhibited lower body core temperature (40 °C vs. 43 °C), lower scrotal temperature (34 °C vs. 36 °C), lower neurological severity scores (2.8 vs. 5.8), higher erectile ability, (5984 mmHg-sec vs. 3788 mmHg-sec), higher plasma testosterone (6.8 ng/mL vs. 3.5 ng/mL), lower plasma follicle stimulating hormone (196.3 mIU/mL vs. 513.8 mIU/mL), lower plasma luteinizing hormone (131 IU/L vs. 189 IU/L), lower plasma adrenocorticotropic hormone (5136 pg/mL vs. 6129 pg/mL), lower plasma corticosterone (0.56 ng/mL vs. 1.18 ng/mL), lower sperm loss and lower values of histopathological scores for epididymis, testis, seminal vesicle, prostate, and bladder. Our data suggest that HBOP reduces body core and scrotal hyperthermia and improves sperm loss, testis/prostate/bladder dysmorphology, and erectile dysfunction after EHS in rats.

中文翻译：

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高压氧预处理使遭受劳力性热损伤的成年雄性大鼠的阴囊温度、精子质量、睾丸结构和勃起功能正常化

在高温环境下工作的男性中已发现睾丸过热的情况。阴囊温度高于正常范围会导致睾丸中的生殖细胞损失，并导致男性生育力低下。在成年雄性大鼠中，在较高环境温度（36°C，相对湿度 50%，52 分钟）下运动会导致劳力性中暑 (EHS)，其特征是阴囊高热、精子质量受损、睾丸、前列腺和膀胱畸形，以及勃起功能障碍。在这里，我们的目的是确定高压氧预处理（HBOP：100％O，2.0 atm绝对[ATA]，每天2小时，持续14天，然后在EHS发病前）是否能够预防EHS引起的不孕、睾丸问题、前列腺和膀胱畸形和勃起功能障碍。在劳累热应激结束时，与常压空气（NBA 或非 HBOP）大鼠相比，HBOP 大鼠表现出较低的身体核心温度（40 °C vs. 43 °C）、较低的阴囊温度（34 °C vs. 36 °C） C)、较低的神经系统严重程度评分（2.8 vs. 5.8）、较高的勃起能力（5984 mmHg-sec vs. 3788 mmHg-sec）、较高的血浆睾酮（6.8 ng/mL vs. 3.5 ng/mL）、较低的血浆卵泡刺激激素（196.3 mIU/mL vs. 513.8 mIU/mL）、较低血浆黄体生成激素（131 IU/L vs. 189 IU/L）、较低血浆促肾上腺皮质激素（5136 pg/mL vs. 6129 pg/mL）、较低血浆皮质酮（0.56 ng/mL vs. 1.18 ng/mL）、精子丢失率较低以及附睾、睾丸、精囊、前列腺和膀胱的组织病理学评分值较低。我们的数据表明，HBOP 可减少 EHS 后大鼠的身体核心和阴囊过热，并改善精子丢失、睾丸/前列腺/膀胱畸形和勃起功能障碍。