The ovulatory response involves diverse molecular determinants, the interplay between which remains less investigated in fish. This study explores the temporal changes in the follicular microenvironment, regulatory factors, and underlying signaling events during ovulation in female zebrafish subjected to 14L:10D at 28 ± 1 °C vis-à-vis in hCG-stimulated full-grown (FG) follicles Congruent with reduced GSH levels, SOD, and GPx activity, a graded increase in follicular free radicals, Nox4, and p38 MAPK phosphorylation in the morning hour groups (05:00 and 06:30) correlates positively with the ovulatory surge in inflammatory mediators (Tnf-α, Il-1β, Il-6, Nos2, and Cox-2). Further, elevated Pgr expression and its nuclear translocation, congruent with follicular and upregulation corroborates well with the transcriptional activation of genes ( triggering ovulation in this species. Mechanistically, an elevated ovulatory response in hCG-treated FG follicles involves the upregulation of inflammatory mediators, and ovulation-associated genes in a manner sensitive to PKA- and MAPK3/1-mediated signaling.

中文翻译：

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卵泡超氧化物、炎症调节剂和内分泌因子在斑马鱼 (Danio rerio) 排卵中的参与：PKA 和 MAPK 信号在 Pgr 排卵标志物调节中的串扰

排卵反应涉及多种分子决定因素，这些因素之间的相互作用在鱼类中的研究仍然较少。本研究探讨了雌性斑马鱼在 28 ± 1 °C 下接受 14L:10D 处理后，与 hCG 刺激的完全生长 (FG) 卵泡相比，卵泡微环境、调节因素和潜在信号事件在排卵期间的时间变化。与 GSH 水平、SOD 和 GPx 活性降低相一致，早晨组（05:00 和 06:30）卵泡自由基、Nox4 和 p38 MAPK 磷酸化逐渐增加，与炎症介质的排卵激增呈正相关。 Tnf-α、Il-1β、Il-6、Nos2 和 Cox-2）。此外，升高的 Pgr 表达及其核易位，与卵泡和上调一致，很好地证实了基因的转录激活（触发该物种的排卵）。从机制上讲，hCG 处理的 FG 卵泡中排卵反应升高涉及炎症介质的上调，并且以对 PKA 和 MAPK3/1 介导的信号传导敏感的方式调节排卵相关基因。