The application of DNA monitoring has recently expanded into the information technology realm of DNA computing and storage systems, leveraging its capabilities for molecular computing and high-density data storage. Essential to this advancement are multi-color fluorescent signals attached to informational DNA, enabling simultaneous multidata reading in the DNA memory or storage system. Various fluorescent detection techniques, such as real-time polymerase chain reaction, next-generation sequencing, and fluorescence resonance energy transfer, have been investigated for DNA reactions and sequence data. Although proximity (less than 10 nm) between different fluorescent signals can lead to interference, controlling the distance of fluorescent molecules attached to DNA is a feasible solution. This study demonstrates a DNA molecular memory system using multiple fluorescent molecules. We examined the independent hybridization of three different fluorescent DNA molecules to DNA templates with three sites for fluorescent attachment on 17 nt DNAs. The study focused on two multi-bit DNA molecules hybridized to the template DNA, assessing their fluorescence emission intensities at various excitation wavelengths. Two multi-bit DNA molecules, which were hybridized onto the template DNA, were investigated for fluorescence emission intensities by various excitation wavelengths. Although the emission intensities of the two multi-bit DNA molecules were not significantly increased by another fluorescent molecule, each excitation wavelength has provided more effective emission intensity levels for DNA signal detection. Furthermore, we developed a three-bit DNA molecular memory system using triple-level DNA molecules. These multi-color DNA systems could be extended to arithmetic and logical computing.

中文翻译：

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多重荧光多位DNA记忆编码系统

DNA监测的应用最近已扩展到DNA计算和存储系统的信息技术领域，利用其分子计算和高密度数据存储的能力。这一进步的关键是附加在信息 DNA 上的多色荧光信号，从而能够在 DNA 内存或存储系统中同时读取多数据。各种荧光检测技术，如实时聚合酶链式反应、下一代测序和荧光共振能量转移，已针对 DNA 反应和序列数据进行了研究。尽管不同荧光信号之间的接近度（小于 10 nm）可能会导致干扰，但控制附着在 DNA 上的荧光分子的距离是一个可行的解决方案。这项研究展示了使用多个荧光分子的 DNA 分子记忆系统。我们检查了三种不同荧光 DNA 分子与 DNA 模板的独立杂交，其中 17 nt DNA 上具有三个荧光附着位点。该研究重点关注与模板 DNA 杂交的两个多位 DNA 分子，评估它们在不同激发波长下的荧光发射强度。研究了与模板 DNA 杂交的两个多位 DNA 分子在不同激发波长下的荧光发射强度。虽然两个多位DNA分子的发射强度没有被另一个荧光分子显着增加，但每个激发波长都为DNA信号检测提供了更有效的发射强度水平。此外，我们还利用三级DNA分子开发了三位DNA分子存储系统。这些多色 DNA 系统可以扩展到算术和逻辑计算。