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The Cys-2088-Arg mutation in the ACCase gene and enhanced metabolism confer cyhalofop-butyl resistance in Chinese sprangletop (Leptochloa chinensis)
Pesticide Biochemistry and Physiology ( IF 4.7 ) Pub Date : 2024-02-10 , DOI: 10.1016/j.pestbp.2024.105826 Min Liao , Minghao Jiang , Xumiao Wang , Wei Hu , Ning Zhao , Haiqun Cao
Pesticide Biochemistry and Physiology ( IF 4.7 ) Pub Date : 2024-02-10 , DOI: 10.1016/j.pestbp.2024.105826 Min Liao , Minghao Jiang , Xumiao Wang , Wei Hu , Ning Zhao , Haiqun Cao
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Acetyl-CoA carboxylase (ACCase)-inhibiting herbicides are among the most commonly used herbicides to control grassy weeds, especially , in rice fields across China. Herein, we collected a suspected resistant (R) population of (HFLJ16) from Lujiang county in Anhui Province. Whole plant dose response tests showed that, compared with the susceptible (S) population, the R population showed high resistance to cyhalofop-butyl (22-fold) and displayed cross-resistance to metamifop (9.7-fold), fenoxaprop--ethyl (18.7-fold), quizalofop--ethyl (7.6-fold), clodinafop-propargyl (12-fold) and clethodim (8.4-fold). We detected an amino acid substitution (Cys-2088-Arg) in the of resistant L. . However, gene expression levels were not significantly different ( > 0.05) between R plants and S plants, without or with cyhalofop-butyl treatment. Furthermore, pretreatment with piperonyl butoxide (PBO, a cytochrome P450 monooxygenase (CYP450) inhibitor) or 4-chloro-7-nitrobenzoxadiazole (NBD-Cl, a glutathione-S-transferase (GST) inhibitor), inhibited the resistance of the R population to cyhalofop-butyl significantly (by approximately 60% and 26%, respectively). Liquid chromatography tandem mass spectrometry analysis showed that R plants metabolized cyhalofop-butyl and cyhalofop acid (its metabolite) significantly faster than S plants. Three CYP450 genes, one GST gene, and two ABC transporter genes were induced by cyhalofop-butyl and were overexpressed in the R population. Overall, GST-associated detoxification, CYP450 enhancement, and target-site gene mutation are responsible for the resistance of L. to cyhalofop-butyl.
更新日期：2024-02-10
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