In addition to the acute lethal toxicity, insecticides might affect population dynamics of insect pests by inducing life history trait changes under low concentrations, however, the underlying mechanisms remain not well understood. Here we examined systemic impacts on development and reproduction caused by low concentration exposures to cyantraniliprole in the fall armyworm (FAW), and the putative underlying mechanisms were investigated. The results showed that exposure of third-instar larvae to LC and LC of cyantraniliprole significantly extended larvae duration by 1.46 and 5.41 days, respectively. Treatment with LC of cyantraniliprole significantly decreased the pupae weight and pupation rate as well as the longevity, fecundity and egg hatchability of female adults. Consistently, we found that exposure of FAW to LC cyantraniliprole downregulated the mRNA expression of four ecdysteroid biosynthesis genes including , , and and one ecdysone response gene in the larvae as well as the gene encoding vitellogenin () in the female adults. We also found that treatment with LC of cyantraniliprole significantly decreased the whole body levels of glucose, trehalose, glycogen and triglyceride in the larvae. Our results indicate that low concentration of cyantraniliprole inhibited FAW development by disruption of ecdysteroid biosynthesis as well as carbohydrate and lipid metabolism, which have applied implications for the control of FAW.

中文翻译：

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低浓度的氰虫酰胺通过破坏蜕皮类固醇生物合成以及碳水化合物和脂质代谢，对草地贪夜蛾的发育产生负面影响

除了急性致死毒性外，杀虫剂还可能通过在低浓度下诱导生活史性状变化来影响害虫的种群动态，但其潜在机制仍不清楚。在这里，我们研究了低浓度氰虫​​酰胺对秋粘虫 (FAW) 发育和繁殖造成的系统性影响，并研究了假定的潜在机制。结果表明，三龄幼虫暴露于 LC 和 LC 氰虫酰胺后，幼虫存活期分别显着延长 1.46 天和 5.41 天。用氰虫酰胺 LC 处理显着降低了蛹重量和化蛹率以及雌性成虫的寿命、繁殖力和卵孵化率。我们一致地发现，秋粘虫接触 LC 氰虫酰胺会下调幼虫中四种蜕皮类固醇生物合成基因的 mRNA 表达，包括 、 和 ，以及一种蜕皮激素反应基因，以及雌性成虫中编码卵黄蛋白原 () 的基因。我们还发现，用LC氰虫酰胺处理显着降低了幼虫体内葡萄糖、海藻糖、糖原和甘油三酯的水平。我们的结果表明，低浓度的氰虫酰胺通过破坏蜕皮类固醇生物合成以及碳水化合物和脂质代谢来抑制秋粘虫的发育，这对控制秋粘虫具有应用意义。