is one of the devastating fungal pathogens of sugarcane, accountable for the red rot pandemic in India. Sucrose, being main assimilatory product of sugarcane, has been accredited for its crucial roles as signaling molecule, and association with biotic responses against pathogens. This is the first study to investigate the effect of infection on sucrose metabolism in stalk tissues of red rot resistant (BO91) and susceptible (CoJ64) cultivars of sugarcane. Our experiment included nanoLCMS/MS-based investigation of peptide abundance of the proteins implicated in sucrose metabolism, followed by qRT-PCR-based validation at gene level. The results indicated that promotes sucrose biosynthesis in CoJ64 cultivar by enhancing the abundance of SPS-1, 4, 12, 14, and SPP-1, 2, 3, 5. In case of invertases, implicated in sucrose degradation, contrasting results of peptide abundance were pragmatic between AIs and ANIs after infection. infection induced the abundance of AI proteins (SAI-1, 2, 3, 8, 9, 12, 14, CWI-2, VI-1), whereas the abundance of ANI proteins was reduced (ANI-1, 4, 6, 10, 11) in BO91 cultivar. Similarly, fungal infection also reduced the peptide abundance of ANIs (ANI-6, 10) in CoJ64 cultivar. Peptide abundance of SuSy proteins, involved in irreversible catalysis of sucrose, was also enhanced by fungal infection in both the cultivars of sugarcane. Results indicated that SuSy-1 and 4 in BO91 cultivar, and SuSy-1 and 6 in CoJ64 cultivar were significantly induced by infection. The results display a positive impact of infection on sucrose accumulation in the stalk tissues of sugarcane.

中文翻译：

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镰状炭疽菌通过调节 SPS、SPP、SuSy 和转化酶的表达来影响甘蔗茎中蔗糖的积累

是甘蔗的毁灭性真菌病原体之一，是印度红腐病大流行的罪魁祸首。蔗糖是甘蔗的主要同化产物，因其作为信号分子的重要作用以及与针对病原体的生物反应的相关性而被认可。这是第一项研究感染对红腐病抗性（BO91）和敏感（CoJ64）甘蔗品种茎组织中蔗糖代谢的影响的研究。我们的实验包括基于 nanoLCMS/MS 的研究，对蔗糖代谢中涉及的蛋白质的肽丰度进行研究，然后在基因水平上进行基于 qRT-PCR 的验证。结果表明，通过提高 SPS-1、4、12、14 和 SPP-1、2、3、5 的丰度，促进 CoJ64 品种中的蔗糖生物合成。对于涉及蔗糖降解的转化酶，与肽的结果对比感染后 AI 和 ANI 之间的丰度是实用的。感染诱导 AI 蛋白丰度 (SAI-1, 2, 3, 8, 9, 12, 14, CWI-2, VI-1)，而 ANI 蛋白丰度减少 (ANI-1, 4, 6, 10, 11) 在 BO91 品种中。同样，真菌感染也降低了 CoJ64 品种中 ANI (ANI-6, 10) 的肽丰度。两个甘蔗品种中，参与蔗糖不可逆催化的 SuSy 蛋白的肽丰度也因真菌感染而增加。结果表明，BO91品种中的SuSy-1和4以及CoJ64品种中的SuSy-1和6受到感染的显着诱导。结果显示感染对甘蔗茎组织中蔗糖积累有积极影响。