The control of infections is one of the key strategies to treat cuts, wounds, lung, and skin infections. In this study the folkloric use of () roots in the mentioned conditions was scientifically investigated. The methanolic (MeOH) crude extract of root was fractionated (n-hexane, ethyl acetate and water) via bioassay-guided method, and its antibacterial activity was evaluated using the agar well diffusion and Minimum inhibitory concentration (MIC) assays against clinical isolate of (). The antibiofilm activity was measured using the crystal violet staining method. The crude extract, fractions and sub-fractions tested showed the MICs values ranging from 200 to 1000 μg/mL respectively. Among the fractions, notably, the water fraction exhibited the highest activity against . The water fraction was then subjected to thin layer chromatography (TLC). Following spectrometric analysis using HPLC-ESI-Q-TOF-MS, gallic acid and emodin were identified as the primary components within the same fraction, responsible for eliciting antibacterial and antibiofilm effects. The in-silico studies conducted with AutoDock Vina on the LasR protein, using both isolated gallic acid and emodin, confirm the binding affinity of these molecules to the active sites of the LasR protein that has regulatory role in building of biofilm formation and its pathogenicity. By scientifically validating the infection-controlling properties of , this research provides compelling evidence that supports its traditional use as reported in folklore. Moreover, this study contributes to our understanding of the plant's potential in managing infections, thereby substantiating its traditional therapeutic application in a scientific context.

中文翻译：

---

通过 HPLC-ESI-Q-TOF-MS 表征酸模根提取物的抗菌和抗菌膜潜力

控制感染是治疗割伤、伤口、肺部和皮肤感染的关键策略之一。在这项研究中，对上述条件下 () 词根的民俗使用进行了科学调查。通过生物测定引导的方法对根的甲醇（MeOH）粗提物进行分级（正己烷、乙酸乙酯和水），并使用琼脂井扩散和针对临床分离的最低抑菌浓度（MIC）测定来评估其抗菌活性。 ()。使用结晶紫染色法测量抗生物膜活性。测试的粗提物、级分和亚级分的 MIC 值分别为 200 至 1000 μg/mL。在这些级分中，值得注意的是，水级分表现出最高的抗 活性。然后将水部分进行薄层色谱(TLC)。使用 HPLC-ESI-Q-TOF-MS 进行光谱分析后，确定没食子酸和大黄素是同一组分中的主要成分，负责引发抗菌和抗生物膜作用。使用 AutoDock Vina 对 LasR 蛋白进行的计算机模拟研究，使用分离的没食子酸和大黄素，证实了这些分子与 LasR 蛋白活性位点的结合亲和力，LasR 蛋白在生物膜形成及其致病性的构建中具有调节作用。通过科学地验证 的感染控制特性，这项研究提供了令人信服的证据来支持民间传说中报道的其传统用途。此外，这项研究有助于我们了解该植物在控制感染方面的潜力，从而在科学背景下证实其传统的治疗应用。