Early detection of prostate cancer, the second main cause of death in men, with robust assay platforms by using the appropriate biomarkers is of great importance for diagnosis and follow-up of disease under treatment. The aim of this research is to investigate how novel TiS nanoribbons can be used as a channel material in the microfluidic electrolyte-gated field-effect transistor (FET), with the goal of developing a label-free immunosensor for the sensitive, selective, and rapid detection of PSA as a cancer marker in both PBS and human serum samples. To create an active channel material, the TiS nanoribbons were deposited onto the FET surface through a drop-casting process, and the surface of the channel was subsequently modified with an anti-PSA monoclonal antibody. The electrical properties of the microfluidic electrolyte-gated TiS nanoribbon-based FET were characterized, and the results showed that it exhibited a depletion-mode n-type behavior with a field-effect mobility of 2.3 × 10 cm/Vs, an I/I current ratio of 4.12, and a subthreshold swing (SS) of 914.1 mV/decade. As the concentration of PSA increased from 0.1 fg/mL to 10 pg/mL, there was a corresponding increase in the drain current with a high sensitivity of 2.2665 nA/decade and a detection limit of 0.04 fg/mL. Integrating the electrolyte-gated FET with the microfluidic channel resulted in improved performance of the microfluidic electrolyte-gated FET immunosensor. The combination of these two components led to better control and delivery of small sample volumes to the surface of the electrolyte-gated FET, which improved the repeatability of the obtained data. Based on the results obtained from the microfluidic immunosensor, it can be inferred that the developed platform has the potential to be an excellent candidate for point-of-care cancer diagnosis and therapeutic monitoring.

中文翻译：

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基于微流体电解质门控 TiS3 纳米带的场效应晶体管作为超灵敏无标记免疫传感器用于前列腺癌标记物分析

前列腺癌是男性第二大死亡原因，通过使用适当的生物标志物，通过强大的检测平台进行早期检测，对于治疗中疾病的诊断和随访非常重要。本研究的目的是研究如何将新型 TiS 纳米带用作微流控电解质门控场效应晶体管 (FET) 中的通道材料，目标是开发一种无标记免疫传感器，用于敏感、选择性和快速检测 PBS 和人血清样本中作为癌症标志物的 PSA。为了创建活性通道材料，通过滴铸工艺将 TiS 纳米带沉积到 FET 表面，随后用抗 PSA 单克隆抗体对通道表面进行修饰。对微流控电解质门控 TiS 纳米带 FET 的电性能进行了表征，结果表明其表现出耗尽模式 n 型行为，场效应迁移率为 2.3 × 10 cm/Vs，I/I电流比为 4.12，亚阈值摆幅 (SS) 为 914.1 mV/十倍频程。随着 PSA 浓度从 0.1 fg/mL 增加到 10 pg/mL，漏极电流相应增加，灵敏度为 2.2665 nA/decade，检测限为 0.04 fg/mL。将电解质门控 FET 与微流体通道集成可提高微流体电解质门控 FET 免疫传感器的性能。这两种成分的结合可以更好地控制和输送小体积样品到电解质门控 FET 的表面，从而提高了所获得数据的可重复性。根据微流控免疫传感器获得的结果，可以推断所开发的平台有潜力成为即时癌症诊断和治疗监测的优秀候选者。