Agricultural soils are the largest anthropogenic source of atmospheric nitrous oxide (NO) that causes global warming and stratospheric ozone depletion. In addition to the well-known emission associated with fertilization, significant NO emission during the harvest season has been reported for soybean fields. Because soybean production is increasing, it is important to understand the production and consumption mechanisms of NO in soybean fields. This study aimed to identify the microbial production processes using the ratios of isotopically substituted molecules, isotopocules. We also investigated the effectiveness of inoculating soybean with symbiotic nitrogen-fixing soil bacteria (rhizobia) with a high NO-reducing ability, which was suggested as a mitigation option for NO emission from soybean fields, on the basis of the characteristic isotope effect during NO reduction.

中文翻译：

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同位素分析揭示大豆田 N2O 产生和还原过程的动态

农业土壤是大气一氧化二氮 (NO) 的最大人为来源，导致全球变暖和平流层臭氧消耗。除了众所周知的与施肥相关的排放外，据报道大豆田在收获季节也有大量的二氧化氮排放。由于大豆产量不断增加，了解大豆田NO的产生和消耗机制非常重要。本研究旨在利用同位素取代分子（同位素子）的比率来确定微生物生产过程。我们还研究了在大豆中接种具有高 NO 还原能力的共生固氮土壤细菌（根瘤菌）的有效性，根据 NO 过程中的特征同位素效应，建议将其作为大豆田 NO 排放的缓解方案。减少。