Biocatalysis has emerged as a powerful technology in recent years to synthesize clinically valuable molecules. In this context, hydrolases have been used to catalyse various organic transformations. Among hydrolases, α-amylase has been recognized as a valuable biocatalyst for chemical processing in a benign reaction environment. Further, it has been widely accepted in natural transformations like hydrolysis of starch as well as in non-natural transformations due to good selectivity, broad substrate scope, and good stability in organic solvents. Several significant organic transformations, such as the Aldol condensation, Hantzsch reaction, Mannich reaction, Knoevenagel condensation, Michael addition, and the multicomponent reaction catalyzed by α-amylase have been covered in this review. Further, the current review notes that α-amylase catalyses not only a single-step reaction but also multi-step and tandem non-natural transformations.

中文翻译：

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有机合成中 α-淀粉酶混杂性的十年更新

近年来，生物催化已成为合成具有临床价值的分子的强大技术。在这方面，水解酶已被用来催化各种有机转化。在水解酶中，α-淀粉酶已被认为是在良性反应环境中进行化学加工的有价值的生物催化剂。此外，由于其良好的选择性、广泛的底物范围和在有机溶剂中良好的稳定性，它在淀粉水解等自然转化以及非自然转化中被广泛接受。本综述涵盖了几个重要的有机转化，例如羟醛缩合、Hantzsch 反应、曼尼希反应、Knoevenagel 缩合、迈克尔加成以及 α-淀粉酶催化的多组分反应。此外，当前的评论指出，α-淀粉酶不仅催化单步反应，而且还催化多步和串联非自然转化。