Histone-deacetylases (HDACs) are epigenetic modulators involved in the control of gene expression. No data are available on the expression or subcellular localization of HDACs in salivary glands. The present study aims to examine the subcellular distribution of HDACs in salivary glands during postnatal development. The major salivary glands of C57/BL6 mice were separately removed at 10, 25, 30,60 and 90 days after birth. Hematoxylin-eosin (H&E) and immunohistochemical staining were performed for HDACs. Gene Expression of HDACs in C57BL/6. NOD-Aec1Aec2 mice salivary glands during the development of Sjögren's syndrome-like illness were also analyzed by using the gene expression datasets (GSE 15640). In the mice salivary gland, HDACs were found to have different localization patterns at various stages of development (10, 25, 30, 60, and 90 days). Apart from HDAC6, ductal cells of salivary glands were the primary sites for HDAC localization. HDAC2, 8, 5, 10 and 11 were expressed at high levels in the salivary gland after birth while HDAC6 showed no expression during postnatal development. This suggests that these HDAC subtypes may have different roles in salivary gland function. In the context of Sjögren's syndrome-like illness, HDAC 2, 8 and 10 showed low expression while HDAC1, 6,5,3 and 11 had relatively high expression in the salivary gland. This study has provided an important reference for understanding the spatiotemporal-specific expression of HDACs in the salivary gland. These results offer new clues for the experimenters and hold promise for developing innovative therapeutic strategies for salivary gland-related diseases.

中文翻译：

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小鼠出生后发育过程中唾液腺中组蛋白脱乙酰酶的免疫定位模式

组蛋白脱乙酰酶 (HDAC) 是参与基因表达控制的表观遗传调节剂。没有关于唾液腺中 HDAC 的表达或亚细胞定位的数据。本研究旨在检查出生后发育过程中唾液腺中 HDAC 的亚细胞分布。C57/BL6小鼠在出生后10、25、30、60和90天分别切除主要唾液腺。对 HDAC 进行苏木精-伊红 (H&E) 和免疫组织化学染色。C57BL/6 中 HDAC 的基因表达。还使用基因表达数据集 (GSE 15640) 分析了 NOD-Aec1Aec2 小鼠在干燥综合征样疾病发展过程中的唾液腺。在小鼠唾液腺中，HDAC 在不同的发育阶段（10、25、30、60 和 90 天）具有不同的定位模式。除 HDAC6 外，唾液腺导管细胞是 HDAC 定位的主要位点。HDAC2、8、5、10和11在出生后唾液腺中高水平表达，而HDAC6在出生后发育过程中不表达。这表明这些 HDAC 亚型可能在唾液腺功能中具有不同的作用。在干燥综合征样疾病的背景下，HDAC 2、8和10在唾液腺中表现出低表达，而HDAC1、6、5、3和11具有相对高的表达。该研究为了解唾液腺中HDACs的时空特异性表达提供了重要参考。这些结果为实验者提供了新的线索，并有望为唾液腺相关疾病开发创新的治疗策略。