Bovine serum albumin, based on its low toxicity and excellent biocompatibility, has great potential as a carrier for drug delivery systems. In this context, a pH/GSH dual-responsive drug delivery system (ICG/DOX@Fe-BSA-FA nanoparticles) based on Bovine serum albumin (BSA) is constructed for tumor-targeted multimodal therapy. In this study, doxorubicin (DOX) and indocyanine green (ICG) are co-loaded after Fe³⁺ is coordinated with BSA, and the system is finally grafted with the targeted molecule Folic acid. DOX is used in chemotherapy, ICG is used in photodynamic therapy (PDT) and photothermal therapy (PTT), and Fe³⁺ mainly provided sufficient raw material for the Fenton-like reaction. The ability of ICG/DOX@Fe-BSA-FA (ID@FBF) nanoparticles to combine chemotherapy with CDT/PDT/PTT is demonstrated by several physical characterization. In vitro cell uptake assay and in vitro cytotoxicity assay demonstrated the excellent ability of ID@FBF to target 4T1 cells and kill tumor cells with the IC₅₀ value of 0.75 µg mL⁻¹. Therefore, chemotherapy combined with CDT/PDT/PTT therapy may result in satisfactory efficacy via ID@FBF, a potentially powerful and highly effective nanosystem.

中文翻译：

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基于牛血清白蛋白纳米颗粒的 pH/GSH 双响应、叶酸靶向给药系统用于癌症治疗

牛血清白蛋白因其低毒性和优异的生物相容性，作为药物输送系统的载体具有巨大的潜力。在此背景下，构建了基于牛血清白蛋白（BSA）的pH/GSH双响应药物递送系统（ICG/DOX@Fe-BSA-FA纳米颗粒），用于肿瘤靶向多模式治疗。^{本研究将Fe 3+}与BSA配位后，将阿霉素（DOX）和吲哚菁绿（ICG）共负载，最终将系统与目标分子叶酸接枝。DOX用于化疗，ICG用于光动力疗法(PDT)和光热疗法(PTT)，Fe ³⁺主要为类Fenton反应提供充足的原料。ICG/DOX@Fe-BSA-FA (ID@FBF) 纳米颗粒将化疗与 CDT/PDT/PTT 相结合的能力已通过多项物理特性得到证明。体外细胞摄取实验和体外细胞毒性实验表明ID@FBF具有优异的靶向4T1细胞和杀伤肿瘤细胞的能力，IC ₅₀值为0.75 µg mL ^-1。因此，化疗联合CDT/PDT/PTT疗法可能会通过ID@FBF这一潜在强大且高效的纳米系统获得令人满意的疗效。