Parkinson's disease (PD) is a common chronic central nervous system disorder that reduces quality of life and increases mortality. Currently, the treatment of PD is limited due to the challenge of macromolecular drugs crossing the blood-brain barrier (BBB). Black phosphorus (BP) has antioxidant and anti-inflammatory effects and shows potential for neuroprotection. To solve the restriction of the BBB, innovative BP microbubbles (BP@MBs) combined with focused ultrasound (FUS) are constructed to facilitate BBB opening. Additionally, the biocompatibility and safety of BP@MBs with varying dimensions are investigated, including BP quantum dot microbubbles (BPQDs@MBs) and BP nanosheet microbubbles (BPNSs@MBs). The findings indicate that low concentrations of BP have good biosafety and that BPQDs@MBs exhibit better biocompatibility than BPNSs@MBs both in vitro and in vivo at the same concentrations. Moreover, a 6-hydroxydopamine (6-OHDA)-induced PD cell model and an animal model are established to verify the anti-PD effects of BPQDs@MBs and BPNSs@MBs. The results confirmed that BPQDs@MBs and BPNSs@MBs have antioxidant effects that reduced neuronal apoptosis and improved the motor symptoms of PD in rats. BPQDs@MBs combined with FUS may be a promising option for the clinical treatment of PD.

中文翻译：

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黑磷微泡联合超声治疗帕金森病

帕金森病 (PD) 是一种常见的慢性中枢神经系统疾病，会降低生活质量并增加死亡率。目前，由于大分子药物穿过血脑屏障（BBB）的挑战，PD的治疗受到限制。黑磷 (BP) 具有抗氧化和抗炎作用，并具有神经保护潜力。为了解决血脑屏障的限制，创新的BP微泡（BP@MBs）结合聚焦超声（FUS）被构建以促进血脑屏障打开。此外，还研究了不同尺寸的BP@MBs的生物相容性和安全性，包括BP量子点微泡（BPQDs@MBs）和BP纳米片微泡（BPNSs@MBs）。研究结果表明，低浓度的BP具有良好的生物安全性，并且在相同浓度下，BPQDs@MBs在体外和体内均表现出比BPNSs@MBs更好的生物相容性。此外，还建立了6-羟基多巴胺（6-OHDA）诱导的PD细胞模型和动物模型来验证BPQDs@MBs和BPNSs@MBs的抗PD作用。结果证实，BPQDs@MBs 和 BPNSs@MBs 具有抗氧化作用，可减少神经元凋亡并改善大鼠帕金森病的运动症状。BPQDs@MBs 联合 FUS 可能是 PD 临床治疗的一个有前景的选择。