Natural polymer-based hydrogels have been widely applied in bone tissue engineering due to their excellent biocompatibility and outstanding ability of drug encapsulation. However, they have relatively weak mechanical properties and lack bioactivity. Hence, we developed a bioactive nanoparticle composite hydrogel by incorporating LAPONITE®, which is an osteo-inductive inorganic nanoparticle. The incorporation of the nanoparticle significantly enhanced its mechanical properties. In vitro evaluation indicated that the nanocomposite hydrogel could exhibit good biocompatibility. Besides, the nanocomposite hydrogel was proved to have excellent osteogenic ability with up-regulated expression of osteogenic markers such as type I collagen (COL-I), runt-related transcription factor-2 (Runx-2) and osteocalcin (OCN). Furthermore, the in vivo study confirmed that the composite nanocomposite hydrogel could significantly promote new bone formation, providing a prospective strategy for bone tissue regeneration.

中文翻译：

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纳米粒子增强微孔甲基丙烯酸酯丝素蛋白水凝胶促进骨再生

天然聚合物基水凝胶因其优异的生物相容性和突出的药物封装能力而在骨组织工程中得到广泛应用。然而，它们的机械性能相对较弱并且缺乏生物活性。因此，我们通过掺入 LAPONITE®（一种骨诱导性无机纳米颗粒）开发了一种生物活性纳米颗粒复合水凝胶。纳米颗粒的加入显着增强了其机械性能。体外评价表明纳米复合水凝胶具有良好的生物相容性。此外，纳米复合水凝胶被证明具有优异的成骨能力，可上调I型胶原（COL-I）、runt相关转录因子-2（Runx-2）和骨钙素（OCN）等成骨标志物的表达。此外，体内研究证实复合纳米复合水凝胶可以显着促进新骨形成，为骨组织再生提供了一种前瞻性策略。