Background. Oxidative stress is involved in pathogenesis of some psychiatric disorders. To examine the role of oxidative stress in the etiopathogenesis of obsessive-compulsive disorder (OCD), we aimed to determine oxidative stress indices, including malondialdehyde (MDA) levels in serum and red blood cells (RBC) membrane, total antioxidant capacity (TAC), serum glutathione (GSH) levels, serum antioxidant vitamins (A and E), and Na⁺/K⁺-ATPase activity, in patients with the mentioned disorder vs. healthy controls. Method. 39 OCD patients diagnosed based on Diagnostic and Statistical Manual of Mental Disorders (DSM-V) and 39 volunteer healthy subjects were included in this study. MDA levels in serum and RBC membrane were measured using fluorometric method. Serum TAC level, serum GSH level, and Na⁺/K⁺-ATPase activity were also measured using spectrophotometric methods. Serum levels of vitamins were calculated by reversed-phase high-performance liquid chromatography (RP-HPLC). Result. There was a significantly higher MDA level in serum () and RBC membrane () of OCD patients compared with those in controls. A significant reduction in vitamin A () and vitamin E () levels was found in OCD patients vs. controls. There was significantly lower activity of erythrocyte membrane Na⁺/K⁺-ATPase in RBC membrane of OCD patients vs. controls (). Conclusion. Our findings indicate significantly higher levels MDA in both serum and RBC membrane, lower levels of serum vitamins A and E, and lower activity of membrane Na⁺/K⁺-ATPase in OCD patients compared to controls. These suggest an imbalance between oxidant and antioxidant factors in OCD patients that might play a fundamental role in the etiopathogenesis of OCD.

中文翻译：

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强迫症中的氧化状态和 Na+/K+-ATP 酶活性：病例对照研究

背景。氧化应激参与一些精神疾病的发病机制。为了研究氧化应激在强迫症（OCD）发病机制中的作用，我们旨在确定氧化应激指数，包括血清和红细胞（RBC）膜中的丙二醛（MDA）水平、总抗氧化能力（TAC）患有上述疾病的患者与健康对照者的血清谷胱甘肽 (GSH) 水平、血清抗氧化维生素（A 和 E）以及 Na ⁺ /K ^{+ -ATP 酶活性。}方法。本研究纳入了根据《精神疾病诊断与统计手册》（DSM-V）诊断的 39 名强迫症患者和 39 名健康志愿者。采用荧光法测定血清和红细胞膜中的MDA水平。还使用分光光度法测量血清TAC水平、血清GSH水平和Na ⁺ /K ^{+ -ATP酶活性。}通过反相高效液相色谱（RP-HPLC）计算维生素的血清水平。结果。血清中 MDA 水平显着升高（）和红细胞膜（）强迫症患者与对照组相比。维生素A显着减少（）和维生素E（）在强迫症患者与对照组中发现水平。与对照组相比，强迫症患者^{红细胞膜 Na +} /K ⁺ -ATP酶活性显着降低）。 结论。我们的研究结果表明，与对照组相比，强迫症患者的血清和红细胞膜中 MDA 水平显着升高，血清维生素 A 和 E 水平降低，膜 Na ⁺ /K ^{+ -ATP 酶活性降低。}这些表明强迫症患者中氧化剂和抗氧化因子之间的不平衡可能在强迫症的发病机制中发挥重要作用。