Peroxisome proliferator–activated receptor β (pparβ) is a key gene–regulating lipid metabolism pathway, but its function in turbot remains unclear. In this study, the CDS of pparβ was cloned from kidney for the first time. The CDS sequence length was 1533 bp encoding 510 amino acids. Structural analysis showed that the pparβ protein contained a C4 zinc finger and HOLI domain, suggesting that the pparβ gene of turbot has high homology with the PPAR gene of other species. The high expression patterns of pparβ, acox, and cpt-1 at high temperatures, as shown through qPCR, indicated that high temperatures activated the transcriptional activity of pparβ and increased the activity of the acox and cpt-1 genes. The expression of acox and cpt-1 was significantly inhibited when pparβ was downregulated using RNAi technology and inhibitor treatments, suggesting that pparβ positively regulated acox and cpt-1 expression at high temperatures and, thus, modulates lipid catabolism activity. These results demonstrate that pparβ is involved in the regulation of lipid metabolism at high temperatures and expand a new perspective for studying the regulation of lipid metabolism in stress environments of teleost.

过氧化物酶体增殖物激活受体β（pparβ）是调节脂质代谢途径的关键基因，但其在大菱鲆中的功能尚不清楚。本研究首次从肾脏中克隆了pparβ的CDS 。 CDS序列长度为1533bp，编码510个氨基酸。结构分析表明，pparβ蛋白含有C4锌指和HOLI结构域，表明大菱鲆的pparβ基因与其他物种的PPAR基因具有较高的同源性。 qPCR 显示，pparβ、acox和cpt-1在高温下的高表达模式表明高温激活了pparβ的转录活性，并增加了acox和cpt-1基因的活性。当使用RNAi技术和抑制剂处理下调pparβ时， acox和cpt-1的表达被显着抑制，这表明pparβ在高温下正向调节acox和cpt-1的表达，从而调节脂质分解代谢活性。这些结果表明pparβ参与高温下脂质代谢的调节，为研究硬骨鱼应激环境下脂质代谢的调节提供了新的视角。