This trial aimed to customize pikeperch (Sander lucioperca) larval nutrition using live feed enrichments based on Chlorella vulgaris and Trachydiscus minutus. Pikeperch larvae were fed with rotifers and Artemia enriched with C. vulgaris and T. minutus during the first 17 days after exogenous feeding (started 4 days post-hatching [dph]) and only Artemia until 20 dph. Larvae were exposed to seven different enrichments: (a) Nannochloropsis occulata (Nanno 3600 reed Mariculture) (Control), (b) C. vulgaris cultured at 20°C in BG-117 medium (BG20), (c) C. vulgaris cultured at 30°C in BG-117 medium (BG30), (d) T. minutus cultured at 15°C (T15), (e) T. minutus cultured at 25°C (T25), (f) C. vulgaris cultured at 20°C in urea medium (U20), and (g) C. vulgaris cultured at 30°C in urea medium (U30). After 20 days, no significant differences were found between treatments on total length, standard length, myomere height, and eye diameter. On the contrary, significant differences were found in larval fatty acid composition after the trial period. Larvae from the BG30 treatment showed a significantly higher concentration of docosahexaenoic acid (5.61%), and larvae from the T25 treatment had a higher concentration of eicosapentaenoic acid 12.95%. Furthermore, larvae from the U20 treatment had a significantly higher arachidonic acid concentration of 0.116%. Overall, regarding essential fatty acid concentration, a significant difference was observed between the control treatment (Nannochloropsis) and the other treatments. No adverse effects were found on growth or survival when Nannochloropsis-enriched live feed was replaced with the other enrichments. This trial's results will help optimize the pikeperch larvae's nutritional requirements and diversify the live feed enrichments used during the first feeding.

中文翻译：

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使用微藻进行活饲料富集用于梭鲈（Sander lucioperca）幼体培养

该试验旨在使用基于小球藻和Trachydiscus minutus的活饲料浓缩物定制梭鲈 ( Sander lucioperca ) 幼鱼营养。在外源喂养后的前 17 天（孵化后 4 天开始 [dph]），用轮虫和富含C. vulgaris 和 T. minutus的丰年虫喂养梭鲈幼虫，并且仅喂养卤虫直到 20 dph。将幼虫暴露于七种不同的富集环境中：(a)微拟球藻（Nanno 3600 芦苇海水养殖）（对照），(b)在 BG-117 培养基 (BG20) 中于 20°C 培养的普通拟球藻，(c) 在 BG-117 培养基 (BG20) 中培养的普通拟球藻30°C BG-117 培养基 (BG30)，(d) 15°C 培养的T. minutus (T15)，(e) 25°C 培养的T. minutus (T25)，(f)培养的C. vulgaris (g) 在尿素培养基 (U20) 中于 20°C 培养，以及 (g)在尿素培养基 (U30) 中于 30°C 培养的寻常念珠菌。 20天后，治疗之间在总长度、标准长度、肌节高度和眼睛直径方面没有发现显着差异。相反，试验期后幼虫脂肪酸组成存在显着差异。 BG30处理的幼虫显示出显着较高浓度的二十二碳六烯酸(5.61%)，而来自T25处理的幼虫显示出较高浓度的二十碳五烯酸12.95%。此外，U20 处理的幼虫的花生四烯酸浓度明显较高，为 0.116%。总体而言，就必需脂肪酸浓度而言，在对照处理（微绿球藻）和其他处理之间观察到显着差异。当用其他浓缩物代替富含微拟球藻的活饲料时，没有发现对生长或存活有不利影响。该试验的结果将有助于优化梭鲈幼虫的营养需求，并使首次喂养期间使用的活饲料丰富化多样化。