Fluorescence interference in Raman spectroscopy is a well-known problem and is especially significant in portable instruments where the availability of a variety of exciting wavelengths is unlikely. Several fluorescence avoidance and mitigation schemes are described in the literature, and implemented by Raman spectrometer manufacturers, but there is no standard method for evaluating the accuracy and repeatability of these schemes. Some test samples shown in instrument descriptions, such as “dark rum” and “sesame seed oil” are not reproducible. Therefore, we propose a set of colored LEGO blocks as “standard” samples for this purpose; they have the attractive properties of being very low cost, rugged, non-toxic, easy to transport and store, and appear to be manufactured using a standard process. This paper shows the Raman spectra of a set of these blocks at different excitation wavelengths, acquired on laboratory instruments, along with their visible–near-infrared spectra. The goal is to qualitatively understand the origins of the observed fluorescence and lay the groundwork for exploring the effectiveness of methods currently implemented on handheld Raman instruments.

中文翻译：

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乐高积木作为“标准”样本，用于评估拉曼光谱中的荧光避免和减轻

拉曼光谱中的荧光干扰是一个众所周知的问题，在不可能提供多种激发波长的便携式仪器中尤其重要。文献中描述了几种荧光避免和减轻方案，并由拉曼光谱仪制造商实施，但没有标准方法来评估这些方案的准确性和可重复性。仪器说明中显示的一些测试样品，例如“黑朗姆酒”和“芝麻籽油”是不可重复的。因此，我们为此目的提出了一组彩色乐高积木作为“标准”样本；它们具有成本非常低、坚固、无毒、易于运输和储存等吸引人的特性，并且似乎是使用标准工艺制造的。本文显示了在实验室仪器上采集的一组这些模块在不同激发波长下的拉曼光谱及其可见光-近红外光谱。目标是定性地了解所观察到的荧光的起源，并为探索目前在手持式拉曼仪器上实施的方法的有效性奠定基础。