Permanent stains such as trichrome have better sensitivity but are time-consuming and the fixative includes toxic mercuric chloride. Thus, a newer modification was tested and found to be a superior, faster and safer staining technique for intestinal parasitic detection. Our study lasted 9 months and a single stool sample was collected from each enrolled patient. We evaluated classical trichrome (T1 – using Schaudinn fixative) with newer modifications, which involved different fixatives with mordant combinations (T2 – acetic acid + hydrated aluminium sulphate, T3 – citric acid + copper sulphate hydrate). Conventional PCR targeting Entamoeba histolytica, Giardia lamblia and Cryptosporidium spp. was taken as the reference. Out of 175 stool samples, 25.1% protozoa were identified by wet mount, 24% by each T1 and T2, 25.7% by T3. Statistically, T3 and T2 had higher sensitivity as compared to T1 and wet mount when PCR was used as reference.

中文翻译：

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改良三色染色剂可更快、更好地检测肠道原生动物寄生虫

永久性染色剂（例如三色）具有更好的灵敏度，但非常耗时，并且固定剂包含有毒的氯化汞。因此，我们测试了一种更新的修改，发现它是一种用于肠道寄生虫检测的更优越、更快和更安全的染色技术。我们的研究持续了 9 个月，从每位入组患者身上采集了一份粪便样本。我们评估了经典三色（T1 – 使用 Schaudinn 固定剂）和更新的修改，其中涉及不同的固定剂与媒染剂组合（T2 – 乙酸 + 水合硫酸铝，T3 – 柠檬酸 + 硫酸铜水合物）。针对溶组织内阿米巴、蓝氏贾第鞭毛虫和隐孢子虫属的常规 PCR。被作为参考。在 175 个粪便样本中，通过湿涂片鉴定出 25.1% 的原虫，通过 T1 和 T2 鉴定出 24%，通过 T3 鉴定出 25.7%。从统计上看，当使用 PCR 作为参考时，T3 和 T2 比 T1 和湿封片具有更高的灵敏度。