Meniere Disease (MD) is a chronic inner ear disorder characterized by vertigo attacks, sensorineural hearing loss, tinnitus, and aural fullness. Extensive evidence supporting the inflammatory etiology of MD has been found, therefore, by using transcriptome analysis, we aim to describe the inflammatory variants of MD. We performed Bulk RNAseq on 45 patients with definite MD and 15 healthy controls. MD patients were classified according to their basal levels of IL-1β into 2 groups: high and low. Differentially expression analysis was performed using the ExpHunter Suite, and cell type proportion was evaluated using the estimation algorithms xCell, ABIS, and CIBERSORTx. MD patients showed 15 differentially expressed genes (DEG) compared to controls. The top DEGs include IGHG1 (p = 1.64 \(\times\) 10⁻⁶) and IGLV3-21 (p = 6.28 \(\times\) 10⁻³), supporting a role in the adaptative immune response. Cytokine profiling defines a subgroup of patients with high levels of IL-1β with up-regulation of IL6 (p = 7.65 \(\times\) 10⁻⁸) and INHBA (p = 3.39 \(\times\) 10⁻⁷) genes. Transcriptomic data from peripheral blood mononuclear cells support a proinflammatory subgroup of MD patients with high levels of IL6 and an increase in naïve B-cells, and memory CD8⁺ T cells.

梅尼埃病 (MD) 是一种慢性内耳疾病，其特征为眩晕发作、感音神经性听力损失、耳鸣和耳闷感。已经发现了支持 MD 炎症病因的大量证据，因此，通过使用转录组分析，我们旨在描述 MD 的炎症变异。我们对 45 名患有明确 MD 的患者和 15 名健康对照者进行了 Bulk RNAseq。根据 IL-1β 基础水平将 MD 患者分为 2 组：高组和低组。使用 ExpHunter Suite 进行差异表达分析，并使用估计算法 xCell、ABIS 和 CIBERSORTx 评估细胞类型比例。与对照组相比，MD 患者显示出 15 个差异表达基因 (DEG)。顶级 DEG 包括IGHG1 ( p  = 1.64  \(\times\)  10 ⁻⁶ ) 和IGLV3-21 ( p  = 6.28  \(\times\)  10 ⁻³ )，支持适应性免疫反应中的作用。细胞因子分析定义了 IL-1β 水平高且IL6上调( p  = 7.65  \(\times\)  10 ⁻⁸ ) 和INHBA ( p  = 3.39  \(\times\)  10 ⁻⁷ )上调的患者亚组基因。来自外周血单核细胞的转录组数据支持 MD 患者的促炎亚组具有高水平的IL6以及幼稚 B 细胞和记忆 CD8 ⁺ T 细胞的增加。