Transfusion of stored red blood cells (RBCs) to patients is a critical component of human healthcare. Following purification from whole blood, RBCs are stored in one of many media known as additive solutions for up to 42 days. However, during the storage period, the RBCs undergo adverse chemical and physical changes that are often collectively known as the RBC storage lesion. Storage of RBCs in additive solutions modified to contain physiological levels of glucose, as opposed to hyperglycemic levels currently used in most cases, reduces certain markers of the storage lesion, although intermittent doses of glucose are required to maintain normoglycemic conditions. Here, we describe an electrically actuated valving system to dispense small volumes of glucose into 100 mL PVC storage bags containing packed RBCs from human donors. The RBCs were stored in a conventional additive solution (AS-1) or a normoglycemic version of AS-1 (AS-1N) and common markers of stored RBC health were measured at multiple time points throughout storage. The automated feeding device delivered precise and predictable volumes of concentrated glucose to maintain physiological glucose levels for up to 37 days. Hemolysis, lactate accumulation, and pH values of RBCs stored in AS-1N were statistically equivalent to values measured in AS-1, while significant reductions in osmotic fragility and intracellular sorbitol levels were measured in AS-1N. The reduction of osmotic fragility and oxidative stress markers in a closed system may lead to improved transfusion outcomes for an important procedure affecting millions of people each year.

中文翻译：

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低血糖策略对红细胞储存优化的转化影响

将储存的红细胞（RBC）输注给患者是人类医疗保健的重要组成部分。从全血中纯化后，红细胞可在一种称为添加剂溶液的介质中保存长达 42 天。然而，在储存期间，红细胞会发生不利的化学和物理变化，通常统称为红细胞储存病变。尽管需要间歇剂量的葡萄糖来维持正常血糖条件，但红细胞在经过修饰以含有生理水平的葡萄糖（与目前在大多数情况下使用的高血糖水平相反）的添加剂溶液中储存，减少了储存损伤的某些标志物。在这里，我们描述了一种电动阀门系统，可将少量葡萄糖分配到 100 mL PVC 储存袋中，其中含有来自人类捐赠者的红细胞。将红细胞储存在传统的添加剂溶液 (AS-1) 或正常血糖版本的 AS-1 (AS-1N) 中，并在整个储存过程中的多个时间点测量储存的红细胞健康状况的常见标记。自动喂食设备提供精确且可预测量的浓缩葡萄糖，以维持生理葡萄糖水平长达 37 天。AS-1N 中储存的红细胞的溶血、乳酸积累和 pH 值在统计上与 AS-1 中测量的值相当，而 AS-1N 中测量的渗透脆性和细胞内山梨醇水平显着降低。封闭系统中渗透脆性和氧化应激标志物的减少可能会改善每年影响数百万人的重要手术的输血结果。