The aggregation of amyloid-β (Aβ) is one of the important pathological markers of Alzheimer's disease. Ruthenium(II) complexes have good stability, low cytotoxicity, a high fluorescence quantum yield, and a good Stokes shift as fluorescent probes. Based on this, we constructed a fluorescent probe for in vivo real-time imaging and inhibition of Aβ-fibril formation using a complex of Ru polypyridine with organic fluorophores (N,N-dimethylaniline) and hydrophobic peptides (KLVFF). DLS and TEM studies have shown that Ru-YH has an inhibitory effect on the fibrotic aggregation of Aβ. Both in vivo and in vitro studies have shown that Ru-WJ and Ru-YH can quickly cross the blood–brain barrier and successfully detect Aβ in early (2.5-month old) transgenic mouse models. In summary, we have explored the potential of Ru complex based biological probes for early diagnosis and inhibition of AD.

中文翻译：

---

开发用于实时监测 Aβ 寡聚物和抑制 Aβ 原纤维形成的 Ru-聚吡啶复合物

β淀粉样蛋白（Aβ）的聚集是阿尔茨海默病的重要病理标志物之一。钌( II )配合物作为荧光探针具有良好的稳定性、低细胞毒性、高荧光量子产率和良好的斯托克斯位移。在此基础上，我们利用Ru聚吡啶与有机荧光团（ N，N-二甲基苯胺）和疏水性肽（KLVFF）的复合物构建了一种用于体内实时成像和抑制Aβ原纤维形成的荧光探针。DLS和TEM研究表明Ru-YH对Aβ纤维化聚集有抑制作用。体内和体外研究均表明，Ru-WJ和Ru-YH可以快速穿过血脑屏障，并在早期（2.5 个月大）转基因小鼠模型中成功检测到 Aβ。总之，我们探索了基于Ru复合物的生物探针在AD早期诊断和抑制方面的潜力。