Introduction. Candida albicans and Staphylococcus aureus are recognized for their development of resistance and biofilm formation. New therapeutic alternatives are necessary in this context. Hypothesis. Etomidate shows potential application in catheters against mixed biofilms of fluconazole-resistant C. albicans and methicillin-resistant S. aureus (MRSA). Aim. The present study aimed to evaluate the activity of etomidate against mixed biofilms of fluconazole-resistant C. albicans and MRSA. Methodology. The action of etomidate against mature biofilms was verified through the evaluation of biomass and cell viability, and its ability to prevent biofilm formation in peripheral venous catheters was determined based on counts of colony forming units (c.f.u.) and confirmed by morphological analysis through scanning electron microscopy (SEM). Results. Etomidate generated a reduction (P<0.05) in biomass and cell viability starting from a concentration of 250 µg ml−1. In addition, it showed significant ability to prevent the formation of mixed biofilms in a peripheral venous catheter, as shown by a reduction in c.f.u. SEM revealed that treatment with etomidate caused substantial damage to the fungal cells. Conclusion. The results showed the potential of etomidate against polymicrobial biofilms of fluconazole-resistant C. albicans and MRSA.

中文翻译：

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依托咪酯对耐氟康唑白色念珠菌和耐甲氧西林金黄色葡萄球菌的混合生物膜具有良好的活性

介绍。 白色念珠菌和金黄色葡萄球菌因其耐药性的发展和生物膜的形成而闻名。在这种情况下，新的治疗替代方案是必要的。假设。依托咪酯显示出在对抗耐氟康唑白色念珠菌和耐甲氧西林金黄色葡萄球菌（MRSA）混合生物膜的导管中的潜在应用。目的。本研究旨在评估依托咪酯对耐氟康唑白色念珠菌和 MRSA 混合生物膜的活性。方法。通过生物量和细胞活力的评估验证了依托咪酯对成熟生物膜的作用，并根据集落形成单位（cfu）的计数确定了其防止外周静脉导管中生物膜形成的能力，并通过扫描电子显微镜进行形态分析证实了依托咪酯对成熟生物膜的作用。 （扫描电镜）。结果。从浓度为250 µg ml -1开始，依托咪酯导致生物量和细胞活力降低（P <0.05） 。此外，它显示出显着的防止外周静脉导管中混合生物膜形成的能力，如cfu SEM的减少所示，这表明依托咪酯治疗对真菌细胞造成了严重损害。结论。结果显示依托咪酯对抗耐氟康唑白色念珠菌和 MRSA 的多种微生物生物膜的潜力。