Abstract

Sleep is an integral part of the life of all living organisms, which is necessary for maintaining health. Recent scientific evidence has established that sleep is a marker for the development of many brain diseases. However, these discoveries are phenomenological, because there are very few methods for studying the sleeping brain. It is obvious that the development of technologies for intravital and real-time monitoring of the sleeping brain of animals will open the door to progress in understanding the restorative mechanisms of sleep and the control of these processes. In this study, we demonstrate a unique approach for simultaneous real-time monitoring of the sleeping brain in freely moving mice. Due to training of mice and careful selection of only those animals in the experiment that can demonstrate sleep, the method allows for effective long-term studies of the brain under natural sleep maintaining optimal conditions that minimize stress in animals. A new proposed method for studying the sleeping brain in real time will allow significant advances in the study of restorative mechanisms of sleep and will stimulate the development of promising smart-sleep technologies for therapy of brain diseases associated with sleep deficit or disturbance.

中文翻译：

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Method for real-time optical brain monitoring in freely moving mice during wakefulness and natural sleep
清醒和自然睡眠期间自由活动小鼠的实时光学脑监测方法

Abstract 抽象的

Sleep is an integral part of the life of all living organisms, which is necessary for maintaining health. Recent scientific evidence has established that sleep is a marker for the development of many brain diseases. However, these discoveries are phenomenological, because there are very few methods for studying the sleeping brain. It is obvious that the development of technologies for intravital and real-time monitoring of the sleeping brain of animals will open the door to progress in understanding the restorative mechanisms of sleep and the control of these processes. In this study, we demonstrate a unique approach for simultaneous real-time monitoring of the sleeping brain in freely moving mice. Due to training of mice and careful selection of only those animals in the experiment that can demonstrate sleep, the method allows for effective long-term studies of the brain under natural sleep maintaining optimal conditions that minimize stress in animals. A new proposed method for studying the sleeping brain in real time will allow significant advances in the study of restorative mechanisms of sleep and will stimulate the development of promising smart-sleep technologies for therapy of brain diseases associated with sleep deficit or disturbance.
睡眠是所有生物体生命不可或缺的一部分，是维持健康所必需的。最近的科学证据表明，睡眠是许多脑部疾病发生的一个标志。然而，这些发现都是现象学的，因为研究睡眠大脑的方法很少。显然，对动物睡眠大脑进行活体实时监测的技术的发展将为理解睡眠的恢复机制和这些过程的控制打开大门。在这项研究中，我们展示了一种独特的方法，可以同时实时监测自由活动的小鼠的睡眠大脑。由于对小鼠进行训练，并在实验中仔细选择那些能够证明睡眠的动物，该方法可以在自然睡眠下对大脑进行有效的长期研究，保持最佳条件，最大限度地减少动物的压力。一种新提出的实时研究睡眠大脑的方法将使睡眠恢复机制的研究取得重大进展，并将刺激有前景的智能睡眠技术的发展，用于治疗与睡眠不足或睡眠障碍相关的脑部疾病。