In vitro antioxidant and anti-inflammatory activities were investigated using a 70% acidic methanol extract of the colored wheat bran, 'Ariheuk.' Active metabolites were identified via metabolomic analysis using multivariate statistical comparisons. The 'Ariheuk' bran extract (ABE) contained a higher total anthocyanin content (0.19 mg C3G/g) than the general wheat bran extract (GBE) (0.01 mg C3G/g). ABE exhibited stronger antioxidant and anti-inflammatory activities than GBE. The mechanism underlying the anti-inflammatory effects of ABE was explored by assessing the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) in RAW 264.7 cells stimulated with lipopolysaccharide (LPS). The crude ABE extract was also partially fractionated into three subfractions (ABE-F1, ABE-F2, and ABE-F3) using preparative liquid chromatography (Prep-LC) to identify the active metabolites. The total anthocyanin content was highest in ABE-F3 (1.91 ± 0.06 mg C3G/g). Among the subfractions, ABE-F2 exhibited the highest antioxidant and anti-inflammatory activities. Several distinct metabolites contributing to the activities of ABE-F2 were identified, including various cyanidin and peonidin derivatives and apigenin derivatives, such as corymboside and schaftoside.

中文翻译：

---

彩色小麦“Ariheuk”麸皮甲醇提取物的抗氧化和抗炎活性

使用彩色麦麸“Ariheuk”的 70% 酸性甲醇提取物研究了体外抗氧化和抗炎活性。使用多变量统计比较通过代谢组学分析鉴定活性代谢物。“Ariheuk”麸皮提取物 (ABE) 的总花青素含量 (0.19 mg C3G/g) 比普通麦麸提取物 (GBE) (0.01 mg C3G/g) 更高。ABE 表现出比 GBE 更强的抗氧化和抗炎活性。通过评估脂多糖 (LPS) 刺激的 RAW 264.7 细胞中诱导型一氧化氮合酶 (iNOS) 和环氧合酶 2 (COX-2) 的表达，探讨了 ABE 抗炎作用的机制。使用制备型液相色谱 (Prep-LC) 将粗 ABE 提取物部分分级为三个亚组分（ABE-F1、ABE-F2 和 ABE-F3），以鉴定活性代谢物。ABE-F3 中总花青素含量最高（1.91 ± 0.06 mg C3G/g）。在这些亚组分中，ABE-F2 表现出最高的抗氧化和抗炎活性。鉴定出几种对 ABE-F2 活性有贡献的不同代谢物，包括各种矢车菊苷和芍药苷衍生物以及芹菜素衍生物，例如伞形花苷和 schaftoside。