Magnesium (Mg) alloys with biodegradability and excellent mechanical properties are in high demand for applications in guided bone regeneration (GBR). However, the clinical application of Mg alloys is hindered by infection risks and limited osteogenesis. Herein, a structure-functional integrated Mg-Ca/Mg-Cu bilayer membrane was rolled at 150 °C through various single-pass reductions by using online heating rolling. The Mg-Cu layer was specifically engineered to exhibit antibacterial properties tailored for gingival tissue, while the Mg-Ca layer was designed to support bone regeneration within the defect cavity. The bilayer membrane demonstrated a flexural yield strength of 421.0 MPa and a modulus of 58.6 GPa, indicating exceptional deformation resistance. Furthermore, it maintained notable structural stability by retaining 86.4% of its volume after 21 days in Hanks' solution. In vitro results revealed that the bilayer membrane exhibited favorable biocompatibility and promoted osteogenesis via the synergetic effect of released Mg and Ca ions. The rapid release of Cu ions and the creation of an alkaline environment further improved antibacterial properties, potentially preventing postoperative infections. Additionally, in an in vivo rat calvarial defect model, the membrane demonstrated its capability to stimulate new bone formation. In summary, the Mg-Ca/Mg-Cu bilayer membrane exhibited outstanding mechanical stability, favorable corrosion rates, extraordinary osteogenic and antibacterial activity simultaneously. Consequently, it holds promise as a robust barrier membrane in GBR applications.

中文翻译：

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可生物降解的 Mg-Ca/Mg-Cu 双层膜，具有增强的机械、成骨和抗菌性能，适用于 GBR 应用

具有生物可降解性和优异机械性能的镁（Mg）合金在引导骨再生（GBR）中的应用有着很高的需求。然而，镁合金的临床应用受到感染风险和有限的成骨作用的阻碍。在此，通过在线加热轧制，通过各种单道次压下，在150℃下轧制出结构-功能一体化的Mg-Ca/Mg-Cu双层膜。Mg-Cu 层经过专门设计，具有适合牙龈组织的抗菌特性，而 Mg-Ca 层则旨在支持缺损腔内的骨再生。双层膜的弯曲屈服强度为 421.0 MPa，模量为 58.6 GPa，具有出色的抗变形能力。此外，它在 Hanks 溶液中放置 21 天后仍保留了 86.4% 的体积，从而保持了显着的结构稳定性。体外结果表明，双层膜表现出良好的生物相容性，并通过释放的镁离子和钙离子的协同作用促进成骨。铜离子的快速释放和碱性环境的创建进一步提高了抗菌性能，有可能预防术后感染。此外，在体内大鼠颅骨缺损模型中，该膜显示出其刺激新骨形成的能力。综上所述，Mg-Ca/Mg-Cu双层膜同时表现出优异的机械稳定性、良好的腐蚀速率、非凡的成骨和抗菌活性。因此，它有望成为 GBR 应用中坚固的屏障膜。