Antimicrobial packaging reduces the extent of microbial contamination; however, conventional antimicrobial packaging, which releases antimicrobial agents into food, may experience rapid agent depletion and can adversely affect food flavors. In this study, a novel photocatalytic antimicrobial nanofiber film embedded with Rose Bengal (RB) dye that generates reactive oxygen species (ROS) in visible light was designed for inactivating microorganisms. The film’s antimicrobial properties under various light intensities and exposure times were evaluated, using Escherichia coli as a test microorganism. The results demonstrated that RB generates singlet oxygen as its principal ROS and has potent antimicrobial effects when incorporated into a film, achieving a 4.4 ± 0.1 log CFU reduction in E. coli after 45 h under a light intensity of 6500 lx. The film’s antimicrobial efficacy was dependent on light intensity, with significant E. coli inactivation occurring above 2000 lx. Overall, the RB-incorporated film effectively inactivates E. coli, providing a promising alternative to conventional antimicrobial packaging methods.

中文翻译：

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嵌入孟加拉玫瑰的抗菌包装膜在可见光照射下灭活大肠杆菌的功效

抗菌包装减少了微生物污染的程度；然而，传统的抗菌包装会将抗菌剂释放到食品中，可能会导致抗菌剂快速耗尽，并对食品风味产生不利影响。在这项研究中，设计了一种嵌入玫瑰红（RB）染料的新型光催化抗菌纳米纤维膜，该染料在可见光下产生活性氧（ROS），用于灭活微生物。使用大肠杆菌作为测试微生物，评估了薄膜在不同光强度和暴露时间下的抗菌性能。结果表明，RB 产生单线态氧作为其主要 ROS，并且当掺入薄膜中时具有有效的抗菌作用，在6500 lx 的光强度下 45 小时后，大肠杆菌中的 CFU 减少了 4.4 ± 0.1 log CFU。该薄膜的抗菌功效取决于光强度，超过 2000 lx 时大肠杆菌会显着失活。总体而言，加入 RB 的薄膜可有效灭活大肠杆菌，为传统抗菌包装方法提供了一种有前景的替代方案。