It is important to evenly increase the amount of scattered pollen per clone for maintaining high genetic variation in clonal seed orchards. It is thus imperative to determine not only the number of male flowers per tree, but also the amount of pollen per male flower in each clone. In this study, the existence of annual variation on the fresh weight of male flowers (FW) and the clonal and annual variation, and ramets’ variation with the same or different tree ages on the weight of pollen per male flower (WP) were investigated using 21 Abies sachalinensis clones for 3 years. The results indicated that there were significant differences in FW and WP among clones each year and the relationships between FW and WP were linearly significant for every year. WP also showed significant annual variation, while the re was also significant variation in ramets. The clonal repeatability regarding WP was 0.37—0.47 for data obtained for 2 or 3 years and the generalized linear mixed models with the random effects of clone, year, and ramet indicated that the effect of clone more strongly affected WP than other effects. These results suggested that WP on A. sachalinensis is a trait that is considerably influenced by clonal characteristics; there is thus a need to characterize this trait in each clone when using seed orchards.

中文翻译：

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大山冷杉每雄花花粉量的变化

均匀地增加每个克隆的分散花粉量对于维持克隆种子果园的高遗传变异非常重要。因此，不仅需要确定每棵树的雄花数量，还要确定每个克隆中每朵雄花的花粉量。本研究探讨了雄花鲜重(FW)的年际变化以及无性系和年际变化以及分株在相同或不同树龄下雄花花粉重(WP)的变化。使用 21高山冷杉克隆3年。结果表明，各年无性系间FW和WP存在显着差异，且各年FW和WP之间呈线性显着关系。WP也表现出显着的年际变化，同时分株也存在显着的变化。对于2年或3年获得的数据，WP的克隆重复性为0.37-0.47，具有克隆、年份和分株随机效应的广义线性混合模型表明，克隆效应对WP的影响比其他效应更强烈。这些结果表明 WP高山猕猴桃是一种受克隆特征影响很大的性状；因此，在使用种子园时，需要表征每个无性系的这一性状。