Abstract

The innovation of ideas concerning the development of a strategy with cost-effective and easy use in mosquito control is recognized as an integral part of public health research. As the Aedes, Culex and Armigeres mosquitoes are considered as a vector for devastating parasites and pathogens, their identification is of primordial significance in the current scenario. The standard conventional methods become challenging in certain circumstances. Here we introduced the applicability of “Foldscope linked Geometric morphometrics” in mosquito taxonomy. MorphoJ Apache License (Version 2.0) and TpsDig232 were used for geometric morphometric analysis. The NucleoSpin® Tissue Kit (Macherey-Nagel) was used to extract the mosquito genomic DNA. The evolutionary distance calculation of respective specimens was performed using the neighbour joining method with the aid of MEGA X. The results from “Foldscope linked Geometric morphometrics” verified using mitochondrial COI gene-based characterization and revealed the presence of Aedes aegypti, Aedes albopictus and Armigeres subalbatus. The superimposition wireframe representation exposed prominent variation between the aforesaid mosquitoes. The principal component analysis exposed three distinct points in morphospace and precisely distinguished the two Aedes and Armigeres genera. The results gathered from this investigation could be executed in mosquito control research in the future to prevent vector-borne diseases.

中文翻译：

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“Foldscope 连接几何形态测量学”的概念在识别伊蚊和棉铃虫媒介方面有多坚定？

摘要

制定具有成本效益且易于使用的蚊虫控制策略的理念创新被认为是公共卫生研究的一个组成部分。由于伊蚊、库蚊和棉蚊被认为是毁灭性寄生虫和病原体的媒介，因此它们的识别在当前情况下具有重要意义。标准的传统方法在某些情况下变得具有挑战性。这里我们介绍了“Foldscope链接几何形态计量学”在蚊子分类学中的应用。MorphoJ Apache License（2.0 版）和 TpsDig232 用于几何形态分析。NucleoSpin® Tissue Kit (Macherey-Nagel) 用于提取蚊子基因组 DNA。借助MEGA X，使用邻接法计算各个样本的进化距离。“Foldscope 连接几何形态计量学”的结果使用基于线粒体 COI 基因的表征进行验证，并揭示了埃及伊蚊、白纹伊蚊和阿蚊的存在亚阿尔巴图斯。叠加线框表示暴露了上述蚊子之间的显着差异。主成分分析揭示了形态空间中的三个不同点，并精确地区分了伊蚊和棉铃虫两个属。这项调查收集的结果可以用于未来的蚊子控制研究，以预防媒介传播的疾病。