Phellodendri Amurensis Cortex (PAC) is a medicinal herb that has been generally used to treat diarrhea and jaundice. In order to comprehensively evaluate the PAC in the main production areas quality, a qualitative and quantitative method with highly effective, sensitive, and reliable was developed. The chemical compositions of PAC were analyzed, and fingerprints were established by ultra‐high‐performance liquid chromatography‐quadrupole time‐of‐flight tandem mass spectrometry (UPLC‐Q‐TOF‐MS). Then, the determination of berberine, canthin‐6‐one, dictamnine, γ‐fagarine, and magnoflorine from PAC samples was simultaneously performed using UPLC‐QQQ‐MS. Furthermore, the chemical components of PAC from different regions were compared and analyzed by combining hierarchical cluster analysis, principal component analysis, and orthogonal partial least squares discriminant analysis. A total of 58 compounds were identified, including 36 alkaloids, four phenylpropanoids, seven terpenoids, four flavonoids and their glycosides, an organic acid compound, and six other components. The fingerprint results show that samples have good similarity. Meanwhile, the content of the five ingredients in different habitats is quite different. By multivariate statistical analysis, 18 batches of PAC could be divided into three categories, and 20 components were identified as differential markers of various origins. A comprehensive method of PAC quality evaluation and chemical composition difference analysis was established, which provided the scientific basis for quality evaluation and further pharmacological mechanism research.

中文翻译：

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超高效液相色谱-四极杆飞行时间串联质谱和超高效液相色谱-三重四极杆质谱对黄柏主产地关键成分的评价

黄柏 (PAC) 是一种药草，通常用于治疗腹泻和黄疸。为了全面评价主产区PAC的质量，建立了高效、灵敏、可靠的定性定量方法。对PAC的化学成分进行了分析，并通过超高效液相色谱-四极杆飞行时间串联质谱（UPLC-Q-TOF-MS）建立了指纹图谱。然后，使用 UPLC-MS/MS 同时测定 PAC 样品中的小檗碱、斑素 6-酮、白鲜碱、γ-法加林和木兰花碱。此外，结合层次聚类分析、主成分分析和正交偏最小二乘判别分析，对不同地区的PAC化学成分进行了比较分析。共鉴定出58种化合物，包括36种生物碱、4种苯丙素、7种萜类化合物、4种黄酮及其苷、1种有机酸化合物和6种其他成分。指纹图谱结果表明样品具有良好的相似性。同时，不同产地这五种成分的含量也有较大差异。通过多元统计分析，将18批次PAC分为3类，鉴定出20个成分作为不同产地的差异标记物。建立了PAC质量评价和化学成分差异分析的综合方法，为质量评价和进一步药理机制研究提供了科学依据。